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Plasmid#80167Purposefluorescent protein splicing reporterDepositorInsertdsRed and GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57-RFP-P2A-MCS
Plasmid#78933PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 R175H
Plasmid#16436DepositorInsertp53 R175H (TP53 Human)
UseTagsExpressionMammalianMutationR175HPromoterCMVAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF2595
Plasmid#142337PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRORC (RORC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBFD1/HBFD1Bad
Plasmid#53364PurposeWill convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.DepositorInsertcbfd1
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-COL1A1
Plasmid#66602PurposeExpresses procollagen 1 alpha 1 as a GFP fusionDepositorInsertCOL1A1 (COL1A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
HPV16 E7 TCR
Plasmid#122728PurposeExpress HPV16 E7 TCRDepositorInsertHPV16 E7 TCR
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2970
Plasmid#144446PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRORC (RORC Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
hprtSAGFP
Plasmid#41594PurposeIn vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequencesDepositorInsertSA-GFP reporter
UseMouse Targeting; Dsb repair reporter substrateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9_sgAAVS1
Plasmid#199213PurposeAll-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.DepositorInsertAAVS1-gRNA
UseCRISPRTagsExpressionMutationPromoterhuman U6Available sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-Puro
Plasmid#115143PurposeSafe harbor site 231 knock-in vector with puromycin resistance selection cassetteDepositorInsertPuromycin
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ValRS-His
Plasmid#124122PurposeBacterial expression plasmid for PURE system componentsDepositorInsertValyl-tRNA ligase (valS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i1
Plasmid#120882PurposeExpresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.DepositorInsertCas12i1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-Gsdmd
Plasmid#80950PurposeExpresses Gsdmd in mammalian cellsDepositorInsertGsdmd (Gsdmd Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)
Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH N90-beta-catenin
Plasmid#86499PurposeMammalian expression of Myc tagged N90 beta-cateninDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsMycExpressionMammalianMutationDeletion of amino acids 1-90; constitutively acti…PromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-RGVG-M6
Plasmid#70172PurposeExpresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNADepositorInsertRGVG-M6 T7 RNA polymerase
UseTagsHisExpressionBacterialMutationPromoterT5/LacAvailable sinceDec. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pMAL-c2X
Plasmid#75286PurposeExpresses proteins in the cytoplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 18, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits