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Plasmid#141184PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFPDepositorHas ServiceCloning Grade DNAInsertS surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
UseTagsSignal peptide from influenza HA and Superfolder …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHLP712
Plasmid#203741PurposeIntegration of OsTIR1(F74A)-3xMyc at NEUT5L locusDepositorInsertOsTIR1
UseCandida albicans integrating vectorTags3xMycExpressionMutationCodon 74 changed from Phe to Ala (F74A)PromoterACT1 (C. albicans)Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)
Plasmid#209080PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expressionDepositorUseTagssfGFPExpressionMammalianMutationA53TPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1499
Plasmid#143505PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBL1 (RBL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-NM23-H1
Plasmid#25000DepositorInsertNM23-H1 (NME1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II-YPet(short)
Plasmid#118714PurposeThe human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control.DepositorInsertDesmoplakin II-YPet(short) (DSP Human)
UseRetroviralTagsYPet(short)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ATF6 (1-373)
Plasmid#27173PurposeFor mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.DepositorInsertATF6 (ATF6 Human)
UseTagsHAExpressionMammalianMutationContains only aa 1-373 (please see depositor comm…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PPP1R12A wt
Plasmid#31669DepositorInsertProtein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-BclXL-Ubc-rtTA
Plasmid#198189PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthBCLXL (BCL2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV Retrograde)
Viral Prep#26966-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#209730PurposeExpresses presynaptic low affinity ratiometric ATP sensorDepositorInsert(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Green Pegassos
Plasmid#163114PurposeExpresses biosensors for pyruvate in mammalian cellsDepositorInsertGreen Pegassos
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_HA-VSVG
Plasmid#212647PurposepiggyBac vector to express HA-VSVGDepositorInsertVSVG
UsePiggybacTagsHA tagExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EC70208
Plasmid#209469PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU6Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC70198
Plasmid#209459PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC70200
Plasmid#209461PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC70201
Plasmid#209462PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1
Plasmid#114022PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell linesDepositorInserteGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only