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pEF6 VPS52-V5-6His
Plasmid#175096PurposeV5 tagged VPS52 is expressed in mammalian cellsDepositorInsertVPS52 (VPS52 Human)
UseTagsV5ExpressionMammalianMutationN666D- please see depositor commentPromoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2
Plasmid#99330PurposeMammalian expression of NRBF2, which associates with the PI3KC3-C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTri
Plasmid#124662PurposeExpresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coilDepositorInsertSpyCatcher002-oTri
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)-12xHRE
Plasmid#172334Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-GFP-C-LACE
Plasmid#133913PurposeBacterial expression of His6-GFP-C-LACEDepositorInsertsuperfolder green fluorescent protein
UseTagsLACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAP4
Plasmid#136079PurposeAcceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.DepositorInsert
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Clomeleon
Plasmid#203723PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentrationDepositorInsertcyan fluorescent protein linked yellow florescent protein
UseTagsExpressionBacterial and MammalianMutationCyan Fluorescent Protein, Topaz Fluorescent Prote…PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
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SeeSaw Reporter (SSR2.0)
Plasmid#50393PurposeSSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.DepositorInsertSSR2.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCoofy55
Plasmid#122007PurposeMammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis10-SumoStar (Life Sensors) and Multi2: dependi…ExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW-Flag-CtIP
Plasmid#71108Purposemammalian expession of Flag-CtlPDepositorInsertCtlP
UseLentiviralTagsFlagExpressionMammalianMutationPlease see Depositor CommentsPromoterTRE promoter, Tet ONAvailable sinceNov. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorTagsExpressionBacterial and MammalianMutationPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
UseTagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xMARS-nGFP
Plasmid#205235PurposeExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-NRF1-HA
Plasmid#34708DepositorInsertNRF1 (NFE2L1 Human)
UseRetroviralTags3XFlag and HAExpressionMammalianMutationPromoterLTRAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
Plasmid#216377PurposeBaculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)DepositorInsertLDL receptor related protein 6 (LRP6 Human)
UseBaculovirusTags9xHis and HA signal sequence-FLAG-3C cleavage sit…ExpressionInsectMutationPromoterPolyhedrin (LRP6)/p10 (MESD)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only
