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Plasmid#112579PurposeExpressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vectorDepositorInsertNLS-tdTomato-NES
UseLentiviralTagsnuclear export sequence and nuclear localization …ExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRM209 LTP (Luciferase tdTomato Puro)
Plasmid#174723PurposeExpresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
pac
UseLentiviral and LuciferaseTagsE2A linker and T2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-R-GECO1
Plasmid#46021PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP
Plasmid#176015PurposeMammalian expression of EGFP. Parton lab clone DFSDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRM210 LGP (Luciferase GFP Puro)
Plasmid#174722PurposeExpresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance geneDepositorInsertsFirefly Luciferase
eGFP
pac
UseLentiviral and LuciferaseTagsE2A linker and F2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDuEx2-NIR-GECO1
Plasmid#113680PurposeBacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1DepositorInsertNIR-GECO1
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRM18 LTN (Luciferase tdTomato Neo)
Plasmid#174721PurposeExpresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
aph
UseLentiviral and LuciferaseTagsE2A linker and P2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-K-GECO1
Plasmid#105865PurposeMammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1DepositorInsertK-GECO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTwist-WIV1-CoV Δ18
Plasmid#164439PurposeEncodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertWIV1-CoV Spike truncated to remove 18 amino acids
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorInsertddGFP A and ddGFP B
UseTagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
single polypeptide FPX biosensor for caspase-1
Plasmid#60885PurposeFPX biosensor for caspase-1DepositorInsertsingle polypeptide FPX biosensor for caspase-1
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ratiometric FPX sensor for ERK kinase activity
Plasmid#60974PurposeFor imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.DepositorInsertddRFP A-WW domain-ERK substrate-ddFP B
UseTagsERK substrate is before ddFP copy B, WW domain is…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
NESRA-DEVD-BNLS
Plasmid#50849PurposeExpresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.DepositorInsertddRFP A and ddRFP B
UseTagsNES sequence LQKKLEELELDE placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
single polypeptide FPX biosensor for caspase-8
Plasmid#60884PurposeFPX biosensor for caspase-8DepositorInsertsingle polypeptide FPX biosensor for caspase-8
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NES-NIR-GECO1
Plasmid#113683PurposeExpress NIR-GECO1 preferentially in neuronsDepositorInsertNES-NIR-GECO1
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL3.1-Erg
Plasmid#182706PurposeExpress Erg enhancer in mammalian cellsDepositorInsertEnhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-K-GECO1
Plasmid#105864PurposeBacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1DepositorInsertK-GECO1
UseTags6-His TagExpressionBacterialMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
RANLS-DEVD-BNES
Plasmid#50840PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
UseTagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only