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pAS_4xMma PylT_FLAG-AcKRS
Plasmid#140021PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-CFL-SN
Plasmid#181740PurposeAAV vector for expressing CFL-SNDepositorInsertCFL-SN
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-RHOA
Plasmid#183836PurposeRepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertRHOA homology arms with mNeonGreen-linker (RHOA Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK0_003
Plasmid#123925PurposeEncodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK systemDepositorInsertCas9-sgRNA destination
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ECT2_sgRNA
Plasmid#183873PurposepX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.DepositorInsertECT2 sgRNA spacer (ECT2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
UseTagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR
Plasmid#195532PurposeCre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpeCas9-HF
Plasmid#192141PurposeExpresses highly specific SpeCas9, and cloning backbone for sgRNADepositorInsertSpeCas9-HF
UseAAVTagsExpressionMammalianMutationSpeCas9-HF (R247A, N415A, S421A)PromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-pAce-Kv2.1PR
Plasmid#195523PurposeGreen fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hsp68-LacZ-Gateway
Plasmid#37843DepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMutationPromoterMouse Hsp68 minimal promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f
Plasmid#181742PurposeAAV vector for expressing CFL-SN and GCaMP6fDepositorInsertCFL-SN-P2A-GCaMP6f
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-pAce-Kv2.1PR
Plasmid#195529PurposeGreen fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1861
Plasmid#205974PurposeFLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-pAce-Kv2.1PR
Plasmid#195535PurposeFlp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1871
Plasmid#205976PurposeU6-JAGBLH010000220 Cas8-HNH DR-BpiI-DRDepositorInsertCas8-HNH DR-BpiI-DR
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1862
Plasmid#205975PurposeFLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas5 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS1517
Plasmid#205980PurposeFLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only
