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Plasmid#103854PurposeFor cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorHas ServiceCloning Grade DNAInsertU6-PspCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lenti-mCherry-G12V-KRAS-IRES-Blast
Plasmid#153336PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cellsDepositorInsertmCherry-G12V-KRAS (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPR Metabolic Gene Knockout Library
Pooled Library#110066PurposeEncodes gRNAs for targeting metabolic genes in CRISPR screens.DepositorUseLentiviralAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H3.3
Plasmid#42632DepositorInsertHuman H3.3 (H3-3A Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Plasmid#100834PurposeAAV expression of ultrasensitive protein calcium sensor from CamKII promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCamKIIAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-smURFP
Plasmid#80349PurposeLentiviral transfer vector under the CMV promoter that expresses smURFP.DepositorInsertsmURFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIJ8
Plasmid#68122PurposeTemperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expressionDepositorInsertsflp recombinase
rhaS
rhaR
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
hyeA3A-BE4max
Plasmid#157944PurposeMammalian expression of hyeA3A-BE4maxDepositorInserthyeA3A-BE4max
UseCRISPRTagsExpressionMammalianMutationN57GPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE:mCherry-p2a- bReaChES
Plasmid#163037PurposeFLiCRE reporter gene for neuronal expressionDepositorInsertmCherry-p2a-bReaChES
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-P2A-copGFP
Plasmid#72485PurposeExpress Luc2 and copGFPDepositorInsertLuc2-P2A-copGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1Available sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2+IMS-HyPer7
Plasmid#136469PurposeMammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsSMAC/DIABLOExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-AavLEA1
Plasmid#53093PurposeAavLEA1 expression (in BL21 DE3 E. coli strain)DepositorInsertAavLEA1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
H2B-mCherry
Plasmid#108880PurposeExpression of human histone2B fused to mCherry (nuclear marker)DepositorInsertH2B-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-enh-MNase
Plasmid#166035PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/enhancer/MNase
UseTagsGSTExpressionBacterialMutationPromoterlac promoter / tac promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GRM5-Tango
Plasmid#66390PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGRM5 (GRM5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV1)
Viral Prep#162377-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GAL4BD-VP16/pDON207
Plasmid#201230PurposeGAL4-VP16 transcription factorDepositorInsertGAL4BD-VP16
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
B95(DE3) ΔA ΔfabR ΔserB
Bacterial Strain#197655PurposeThis strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.DepositorBacterial ResistanceNoneAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only