pNL-CEF-CD79B-YFP
(Plasmid
#187006)
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PurposeExpression of human CD79B as YFP fusion protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187006 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNL-CEF
- Backbone size w/o insert (bp) 9785
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD79B
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SpeciesH. sapiens (human)
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Insert Size (bp)1425
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Entrez GeneCD79B (a.k.a. AGM6, B29, IGB, Igbeta)
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNL-CEF-CD79B-YFP was a gift from Hermann Eibel (Addgene plasmid # 187006 ; http://n2t.net/addgene:187006 ; RRID:Addgene_187006) -
For your References section:
BAFFR activates PI3K/AKT signaling in human naive but not in switched memory B cells through direct interactions with B cell antigen receptors. Sevdali E, Block V, Lataretu M, Li H, Smulski CR, Briem JS, Heitz Y, Fischer B, Ramirez NJ, Grimbacher B, Jack HM, Voll RE, Holzer M, Schneider P, Eibel H. Cell Rep. 2022 Jun 28;39(13):111019. doi: 10.1016/j.celrep.2022.111019. 10.1016/j.celrep.2022.111019 PubMed 35767961