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Showing: 1041 - 1060 of 147311 results

  1. pFETCh_SSRP1

    Plasmid
    #72370
    Purpose
    Donor vector for 3' FLAG tag of human SSRP1
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    SSRP1 Homology Arms (SSRP1 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72370-plasmid-map-sequence-id-132120

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  2. PX458_SSRP1_1

    Plasmid
    #72371
    Purpose
    Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    SSRP1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72371-plasmid-map-sequence-id-132121

    Enlarge

  3. PX458_SSRP1_2

    Plasmid
    #72372
    Purpose
    Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    SSRP1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72372-plasmid-map-sequence-id-134668

    Enlarge

  4. pFETCh_TGIF2

    Plasmid
    #72373
    Purpose
    Donor vector for 3' FLAG tag of human TGIF2
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    TGIF2 Homology Arms (TGIF2 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72373-plasmid-map-sequence-id-132123

    Enlarge

  5. PX458_TGIF2_1

    Plasmid
    #72374
    Purpose
    Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    TGIF2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72374-plasmid-map-sequence-id-134714

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  6. PX458_TGIF2_2

    Plasmid
    #72375
    Purpose
    Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    TGIF2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72375-plasmid-map-sequence-id-132125

    Enlarge

  7. pJK678

    Plasmid
    #72376
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only
    72376-plasmid-map-sequence-id-138130

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  8. pJK683

    Plasmid
    #72378
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72378-plasmid-map-sequence-id-134010

    Enlarge

  9. pJK694

    Plasmid
    #72379
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Availability
    Academic Institutions and Nonprofits only

  10. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

    Enlarge

  11. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    T. J. Kappock
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72381-plasmid-map-sequence-id-134006

    Enlarge

  12. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

    Enlarge

  13. pJK034

    Plasmid
    #72383
    Purpose
    Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Insert
    phosphoribosylglycinamide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72383-plasmid-map-sequence-id-136200

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  14. pJK515

    Plasmid
    #72385
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylglycinamide formyltransferase 2
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72385-plasmid-map-sequence-id-133919

    Enlarge

  15. pJK592

    Plasmid
    #72386
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72386-plasmid-map-sequence-id-133922

    Enlarge

  16. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

    Enlarge

  17. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

    Enlarge

  18. pJK630

    Plasmid
    #72389
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsA-insV2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72389-plasmid-map-sequence-id-133978

    Enlarge

  19. pJK631

    Plasmid
    #72390
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsAH6-insA2), C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    inserted Ala2 to facilitate cloning
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72390-plasmid-map-sequence-id-133987

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  20. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72391-plasmid-map-sequence-id-133988

    Enlarge

Showing: 1041 - 1060 of 147311 results