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Plasmid#162657PurposeExpresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertBFLF1
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro2-H2B-iRFP
Plasmid#197352PurposeEncoding nuclear-targeted iRFP713.DepositorInsertiRFP with nucleus-targeting sequence
UseLentiviralTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B-GFP
Plasmid#116869PurposeNuclear localized GFPDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL_U2AF1_S34F_mCherry
Plasmid#84019Purposeexpression of mutant U2AF1 in mammalian cellsDepositorInsertU2AF1 (U2AF1 Human)
UseLentiviralTagsFLAG and T2A-mCherryExpressionMammalianMutationS34FPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SC
Plasmid#224552PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsTwin-Strep-HAExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SN
Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsHA-Twin-StrepExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
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hCas9-VPR
Plasmid#68497Purposenuclease competent SP-Cas9 fused to VPRDepositorInsertSP-Cas9-VPR
UseTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
cr3Vn
Plasmid#184861Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoCjCas9-BlastR
Plasmid#194059PurposeMammalian expression of evoCjCas9 nucleaseDepositorInsertevoCjCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP943
Plasmid#104157PurposeBacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fab specific nanobody TP886
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Thy1.1 DEST
Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Dronpa-Kras
Plasmid#182329PurposePlasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertDronpa-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-StagRFP-EKAR-EV
Plasmid#173019PurposeRed/far-red FRET-based ERK activity reporter for multiplexed imaging applications.DepositorInsertsmURFP-StagRFP-EKAR-EV
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF HaloTag-SYB2
Plasmid#197282PurposeEncodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseTagsExpressionMammalianMutationQ76V and F77WPromoterEF1alphaAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B tdTomato
Plasmid#116870PurposeNuclear localized tdTomatoDepositorInsertH2B tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec611
Plasmid#130872PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationI777K, L900SPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only