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Addgene
Showing: 10521 - 10540 of 14149 results

  2. mVenus-hCOL1A1

    Plasmid
    #140111
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    140111-plasmid-map-sequence-id-296751

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  3. mApple-hCOL1A1

    Plasmid
    #140113
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    140113-plasmid-map-sequence-id-296772

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  4. pCEP4_HA-Halo-cmyc-GRM3 C127A

    Plasmid
    #140458
    Purpose
    Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Halo, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140458-plasmid-map-sequence-id-292298

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  7. dFP-mini

    Plasmid
    #120543
    Purpose
    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.
    Depositor
    Brian Chow
    Article
    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/
    Insert
    de novo fluorescence-activating protein mini (dFP-mini)
    Use
    Tags
    6xHis - TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    120543-plasmid-map-sequence-id-234163

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  11. pGBW-m4046755

    Plasmid
    #145573
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145573-plasmid-map-sequence-id-310622

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  12. pGBW-m4046734

    Plasmid
    #145576
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145576-plasmid-map-sequence-id-287753

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  13. pGBW-m4046841

    Plasmid
    #145578
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 helicase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145578-plasmid-map-sequence-id-386453

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  14. pGBW-m4046897

    Plasmid
    #145579
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145579-plasmid-map-sequence-id-353415

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  15. pGBW-m4046935

    Plasmid
    #145580
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145580-plasmid-map-sequence-id-287757

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  16. pGBW-m4046725

    Plasmid
    #145581
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145581-plasmid-map-sequence-id-287758

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  17. pGBW-m4046761

    Plasmid
    #145582
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145582-plasmid-map-sequence-id-287759

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  18. pGBW-m4046777

    Plasmid
    #145583
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145583-plasmid-map-sequence-id-287760

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  19. pGBW-m4046852

    Plasmid
    #145584
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145584-plasmid-map-sequence-id-292508

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  20. pGBW-m4046791

    Plasmid
    #145586
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145586-plasmid-map-sequence-id-287763

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Showing: 10521 - 10540 of 14149 results