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Pooled Library#136986PurposeExpresses sgRNAs targeting human genesDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only
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pVDL9.3
Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-mCherry
Plasmid#75161PurposeDerived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
GoldenBraid 2.0 Kit
Plasmid Kit#1000000076PurposeModular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional unitsDepositorAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen
Plasmid#182387PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen fusionDepositorInsertVNp-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABEhygroHigheGFPhTERT
Plasmid#28169DepositorInserteGFPhTERT
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
p45sheLL
Plasmid#37323DepositorInsertHPV45L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
hRAP-pEZT-BM
Plasmid#177986PurposeExpression human RAP containing a C-terminal 6xHis tag in mammalian cellsDepositorInsertLDL receptor related protein associated protein 1 (LRPAP1 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized
Plasmid#172345PurposeExpression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimizedPromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
WT GLUT1-GFP
Plasmid#200093PurposeWT human GLUT1 with a C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-FLAG-AKAP-Lbc
Plasmid#67571PurposeExpresses GFP and FLAG-tagged AKAP-Lbc in mammalian cellsDepositorInsertA-kinase anchoring protein Lbc (AKAP13 Human)
UseTagsFLAG and GFPExpressionMammalianMutationR2604G in AKAPPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(120nm)-Cys
Plasmid#91572PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP-NRI
Plasmid#23999DepositorInsertNR1 (Grin1 Rat)
UseTagsNR1 signal sequence (aa1-20) and SEP (supereclipt…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG
Plasmid#85225PurposeExpresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsUseAAV and Cre/LoxTagsExpressionMutationPromoterhSynAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
UseTagsExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PHP.AX
Plasmid#195218PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)DepositorInsertSynthetic construct isolate AAV-PHP.AX VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV9cap
Plasmid#198016PurposeFor high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV9 cap
UseAAVTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smMyc-4x6T-WPRE
Plasmid#196415PurposeAstrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smMyc
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-mEos2
Plasmid#168515PurposeExpression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rateDepositorInsertmito-mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only