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We narrowed to 14,773 results for: LEA

Showing: 10461 - 10480 of 14773 results

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pJExpress414_GmPOPB-R663Q

Plasmid

#92240

Purpose

Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663Q mutant

Use

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Glutamine

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698A

Plasmid

#92237

Purpose

Express H698A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698A mutant

Use

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Alanine

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663A

Plasmid

#92238

Purpose

Express R663A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663A mutant

Use

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Alanine

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtK139A

Plasmid

#92403

Purpose

hQPRTase harbouring K139A mutation

Depositor

James Naismith, Huanting Liu

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV cleacable 6xHis

Expression

Bacterial

Mutation

K139A

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TryX

Plasmid

#89623

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Tryparedoxin

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA8

Plasmid

#83873

Purpose

Gateway donor vector for IAA8

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA8 (IAA8 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA16

Plasmid

#83854

Purpose

Gateway donor vector for IAA16

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA16 (IAA16 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE63

Plasmid

#79445

Purpose

recipient plasmid [single nuclease]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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4QL1

Plasmid

#61942

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

WDR5:APC041-G05:C36415 (WDR5 Human)

Use

Tags

6x His and TEV cleavage site

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

March 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAGM1467

Plasmid

#50298

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + HIS tag + enterokinase cleavage site

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-DKFZP566E144

Plasmid

#31593

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

small fragment nuclease (REXO2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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GMIP

Plasmid

#32046

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

GMIP (GMIP Human)

Use

Tags

His (with TEV cleavage)

Expression

Bacterial

Mutation

amino acids 80-357 only

Promoter

Available since

Jan. 13, 2012

Availability

Industry, Academic Institutions, and Nonprofits
Sgf73(1-106)/PET32a

Plasmid

#26962

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sgf73(1-106) (SGF73 Budding Yeast)

Use

Tags

His6 and Trx

Expression

Bacterial

Mutation

with TEV site to cleave the N-terminal his+Trx tag

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGEM-7Zf-wild

Plasmid

#23160

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Ribonuclease assay sub1

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pGEM-7Zf-deleted

Plasmid

#23161

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Ribonuclease assay sub2

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
CJ47 (U1 snRNA (U1;21d) 3G mutant)

Plasmid

#17776

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

U1 snRNA (U1;21d) (snRNA:U1:21D Fly)

Use

Tags

Expression

Mutation

3G mutant

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
M15 pCS-twhh-nBeta gal

Plasmid

#17152

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Use

Tags

nuclear beta-gal

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCpSRb-4

Plasmid

#13726

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Circularly permuted SRβD210K183 with Precission p…

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.MJ0130m

Plasmid

#11528

Depositor

Sung-Hou Kim

Article

Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

Insert

Type I restriction-modification enzyme (MJ_0130m M. jannaschii)

Use

Tags

HisTag, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pLIC.B4.TM1717

Plasmid

#11532

Depositor

Sung-Hou Kim

Article

Shin et al Proc Natl Acad Sci U S A. 2004 Sep 7. 10

Insert

YjeQ GDP binding protein (TM1717 T. maritima)

Use

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only