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Plasmid#115442PurposeConstitutive lentiviral expressionDepositorInsertRBFOX1 (RBFOX1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn1.Twitch2B.WPRE.SV40
Plasmid#100040PurposeAAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signalingDepositorHas ServiceAAV1InsertTwitch2B
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_CELF1
Plasmid#106100PurposeInducible gene expression of EGFP - CELF1DepositorInsertCELF1 (CELF1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-21 Reporter
Plasmid#71870PurposeMammalian expression vector for the analysis of miR-21 activityDepositorInsertReverse complementary sequence of miR-21
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc
Plasmid#208382PurposeDerived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.DepositorTypeEmpty backboneUseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-ARID1A 1759-2285
Plasmid#39476DepositorInsertARID1A (ARID1A Human)
UseTagsHis and V5ExpressionMammalianMutationexpresses ARID1A aa1759-2285PromoterAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
B1 Integrin
Plasmid#186137PurposeFor expression of recombinant beta1 integrin cytosolic domain in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP and untranslated His6 after stop codonExpressionBacterialMutationPromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FAM20C:V5
Plasmid#63584PurposeFAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.DepositorInsertFAM20C (Fam20c Mouse)
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-BtW-eSpCas9
Plasmid#133358PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsFlag-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF1l
Plasmid#214817PurposeBacterial expression of GST-tagged mouse GTSF1lDepositorInsertGTSF1l (Gtsf1l Mouse)
UseTags6x His and GSTExpressionBacterialMutationPromoterCspAAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-GFPL10
Plasmid#98704PurposeCre-dependently express GFPL10 fusion using Introvert SwitchDepositorInsertGFPL10
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationL10: 2609 C->T to remove EcoRI sitePromoterEF1aAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3L
Plasmid#126588PurposeDNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3L (DNMT3L Human)
UseCRISPRTags3XFLag-NLS-dCas9-DNMT3L-NLSExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG -YWAA-pGK-HYG
Plasmid#107381PurposeMammalian expression of FLAG-YWAADepositorInsertcereblon (CRBN Human)
UseTagsFLAGExpressionMammalianMutationCRBN-Y383A/W385APromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer4
Plasmid#138576PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752DepositorInsertCd36 Promoter (Cd36 Mouse)
UseLuciferaseTagsExpressionMutationPromoterMinimal promoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458_Ruby
Plasmid#110164PurposeCas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNADepositorInserthSpCas9
UseCRISPRTags3xFLAG and Ruby2ExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1YFP8
Plasmid#41669DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; 8 C-terminal-m…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorInsertM(BRCT)TdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_FH-AGO2-WT
Plasmid#92006PurposeExpresses FLAG-HA-AGO2 (WT)DepositorInsertAGO2 (AGO2 Human)
UseTagsFLAG-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S197A
Plasmid#131522PurposeExpresses MKP-3 with S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 197 to AlaninePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only