pcDNA3.1-FAM20C:V5
(Plasmid #63584)

• Purpose: FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.
• Depositing Lab: Kenneth Irvine
• Publication: Ishikawa et al PLoS One. 2012;7(8):e42988. doi: 10.1371/journal.pone.0042988. Epub 2012 Aug 10.

Backbone

• Vector backbone: pcDNA3.1/V5-HisB
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FAM20C
• Alt name: family with sequence similarity 20, member C
• Species: M. musculus (mouse)
• Insert Size (bp): 1900
• Entrez Gene: Fam20c (a.k.a. C76981, DMP-4, DMP4, GEF-CK, mKIAA4081)
• Promoter: CMV
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length mouse FAM20C extending 98 bp upstream of the ATG and excluding the stop codon, was amplified from FAM20C mouse BC025826 cDNA by PCR using primers FAM20C-HindIII-F and FAM20C-NotI-R and the resulting fragment was cloned into HindIII and NotI cut pcDNA3.1/V5-His-B to create above plasmid.