Addgene

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pGGEV_3'_Linker

Plasmid

#49289

Purpose

Golden Gate entry vector at position 3, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4-R3_HSPter-RPS5A-NLS

Plasmid

#193371

Purpose

nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3

Plasmid

#203460

Purpose

jAspSnFR3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al Elife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

Plasmid

#132927

Purpose

pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Insert

humanized SpCas9

Use

CRISPR and Synthetic Biology; Pmagic gateway entr…

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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NK639

Plasmid

#109102

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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NK640

Plasmid

#109103

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK642

Plasmid

#109101

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK591

Plasmid

#109080

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mTFP1

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK586

Plasmid

#109069

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_05705

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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NK613

Plasmid

#109091

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry_SV40 NLS

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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NK648

Plasmid

#109104

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

3'UTR EFL

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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NK597

Plasmid

#109086

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

tdTomato

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK595

Plasmid

#109083

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

EGFP

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK585

Plasmid

#109079

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK609

Plasmid

#109078

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK588

Plasmid

#109077

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

pActAvrII_nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Eliminate NcoI site in the promoter

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK606

Plasmid

#109076

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK587

Plasmid

#109075

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK583

Plasmid

#109072

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_10057

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK600

Plasmid

#109068

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_02256

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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