Addgene

We narrowed to 2,255 results for: PRK

Showing: 1021 - 1040 of 2255 results

Results per page:

Sort results by:

pBabe-Puro-MEK-DD

Plasmid

#15268

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

Map2k1 (Mapk1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S218D S222D

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

Plasmid

#65418

Purpose

AAV packaging vector for CamKIIa driven KOR DREADD expression

Depositor

Bryan Roth

Article

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Insert

CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Expression

Mutation

Promoter

CaMKIIa

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag PKN1

Plasmid

#20586

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKN1 (PKN1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PKN2

Plasmid

#20587

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKN2 (PKN2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
MAPK1 gRNA (BRDN0001149093)

Plasmid

#77788

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK1 gRNA (BRDN0001147355)

Plasmid

#77787

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p38 WT O/E (MAPK14)-pcw107-V5

Plasmid

#64624

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK14 (transcript variant 2) (MAPK14 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
shERK2b-mlpx-puro

Plasmid

#65230

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK1 gRNA (BRDN0001147498)

Plasmid

#77789

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
shERK2a-mlpx-puro

Plasmid

#65229

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
shERK1a-mlpx-puro

Plasmid

#65228

Purpose

encodes a shRNA against ERK1

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MAPK14_WT

Plasmid

#82298

Purpose

Gateway Donor vector containing MAPK14, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK14 (MAPK14 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ERK1C202

Plasmid

#164647

Purpose

Bacterial expression plasmid for His6-ERK1C202

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

ERK1C202 (MAPK1 Human)

Use

Tags

His6-tag

Expression

Bacterial

Mutation

K71R/C82S/C144S/C178S/C183S/T202C/C271S

Promoter

T7

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
shERK2b-mlpx-neo

Plasmid

#65231

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
ERK1C82C202

Plasmid

#164650

Purpose

Bacterial expression plasmid for His6-ERK1C82C202

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

ERK1C82C202 (MAPK1 Human)

Use

Tags

His6-tag

Expression

Bacterial

Mutation

K71R/C144S/C178S/C183S/T202C/C271S

Promoter

T7

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MK01_HUMAN_D0

Plasmid

#79713

Purpose

This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Nicholas Levinson, Markus Seeliger, John Chodera

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK01 (MAPK1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CRaf-IRES-pErk reporter

Plasmid

#226170

Purpose

Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)

Use

Tags

3xFlag-tdMCP and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sh2g-ZMYND8-human

Plasmid

#201406

Purpose

Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

Insert

ZMYND8 (ZMYND8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-Nix-S212D

Plasmid

#197515

Purpose

Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-212. Based on Myc-Nix (#100795)

Depositor

Joseph Gordon

Article

da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

Insert

BCL2 interacting protein 3 like (BNIP3L Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Serine 212 changed to aspartic acid

Promoter

CMV

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-Nix-S212A

Plasmid

#197514

Purpose

Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-212. Based on Myc-Nix (#100795)

Depositor

Joseph Gordon

Article

da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

Insert

BCL2 interacting protein 3 like (BNIP3L Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Serine 212 changed to alanine

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge