-
Plasmid#226500PurposeUltrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate releaseDepositorInsertiGluSnFR3 S72T
UseTagsIgK, Myc tag, and PDGFR Beta TM domainExpressionMammalianMutationS72TPromoterCMVAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3-C-GFP-NLS
Plasmid#222604PurposeExpression of MAC3-tagged GFP-NLS (C-terminal)DepositorInsertEGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 6, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-N-GFP-NES
Plasmid#222606PurposeExpression of MAC3-tagged GFP-NES (N-terminal)DepositorInsertEGFP and 3' nuclear exclusion sequence (NES)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-N-GFP-NLS
Plasmid#222607PurposeExpression of MAC3-tagged GFP-NLS (N-terminal)DepositorInsertEGFP and 3' nuclear localizaition sequence (NLS)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-C-GFP-NES
Plasmid#222603PurposeExpression of MAC3-tagged GFP-NES (C-terminal)DepositorInsertEGFP and 5' nuclear exclusion sequence (NES)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-SnoopTag-AffiHER2-SpyTag
Plasmid#216280PurposeExpresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expressionDepositorInsertSnoopTag-AffiHER2-SpyTag
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, SpyTag, and Thrombin cleavage sit…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)
Plasmid#211863PurposeNuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAExpressionMutationC492APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-GFP
Plasmid#205724PurposeExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylaseDepositorInsertpOTC-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHM1_ARATH
Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHNP_ARATH
Plasmid#204238PurposeBacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHNP_ARATH (AT5G58330 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes not include the chloroplastic transit sequen…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
UseTags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF mito-PAcGFP
Plasmid#188905PurposeExpresses mitochondrial photoactivatable GFPDepositorInsertmitochondrial photoactivatable GFP
UseTagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP)
Plasmid#172339PurposemnucTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1_CSL3
Plasmid#200173PurposeAmino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vectorDepositorInsertCSL3
UseTags6x His tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1_SUL-I
Plasmid#200172PurposeAmino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vectorDepositorInsertSUL-I
UseTags6x His tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_sGFP
Plasmid#191561PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and sGFPExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mRuby3
Plasmid#191744PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mRuby3ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mNeptune2.5
Plasmid#191748PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mNeptune2.5ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
Plasmid#192113PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnR8-10
Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only