pET24a MDHM1_ARATH
(Plasmid #204236)

• Purpose: Bacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pET24a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5271
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MDHM1_ARATH
• Alt name: Arabidopsis mitochondrial MDH
• Alt name: amMDH2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 978
• Mutation: Does done include the first 52 residues that are the mitochondrial targeting peptide. A Met is inserted for the start codon.
• GenBank ID: NP_171936.1
• Entrez Gene: mMDH1 (a.k.a. AT1G53240, mitochondrial malate dehydrogenase 1)
• Promoter: Lac
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET24a MDHM1 ARATH SnapGene
  • MCC Clone Information Sheet - MDHM1_ARATH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/Q9ZP06/.

Required for carbon dioxide and energy partitioning in leaves. May limit photorespiration during the dark phase. Its activity is essential to shuttle reductants out of the mitochondria to support photorespiration.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.