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Plasmid#34877DepositorInsertPeft-3 GFP H2B tbb-2 UTR
UseWorm targetingTagsExpressionMutationS65CPromotereef-1A.1 (eft-3)Available sinceFeb. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP3 CKK
Plasmid#59033Purposeexpresses CKK domain of CAMSAP3 in E. coliDepositorInsertCAMSAP3 CKK domain (CAMSAP3 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa K44A
Plasmid#22197DepositorInserthuman Dynamin 1aa K44A (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)/Bla
Plasmid#201557PurposeEcdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc and FLAG-EGFPExpressionMammalianMutationPromoterEF1a and pIMD(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZIF-NLS-EGFP(Y66F)-HOTag6
Plasmid#208749Purposepart B of nls-SparkDrop with non-fluorescent eGFPDepositorInsertZIF-NLS-EGFP(Y66F)-HOTag6
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA, EGFP Spastin M87 with exon 4, N386C
Plasmid#167543PurposeFor doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.DepositorInsertSpastin M87 with exon 4 (SPAST Human)
UseTagsEGFP and HA tagExpressionMammalianMutationN386CPromoterCMVAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4-IRES-EGFP
Plasmid#153948PurposepiggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFPDepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-DD-W/Bla
Plasmid#201560PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DDExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(K42M)
Plasmid#165440PurposeExpresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.DepositorInsertmEGFP(A206K)-paCaMKII(K42M)
UseTagsmEGFP(A206K)ExpressionMammalianMutationK42M: Kinase dead mutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV(gRNA)-CMV-eGFP-U6(sgCTG)
Plasmid#216732PurposeContains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytesDepositorArticleInsertsgCTG lentiviral guide RNA with eGFP tag
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-GFP-Tdtomato-iRFP670
Plasmid#111812PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
Tdtomato
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
Plasmid#69100PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFPDepositorInsertsyZ3EV cassette
Z3 promoter
yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
1099 pcDNA GFP FKHR AAA
Plasmid#9023DepositorInsertFKHR AAA (FOXO1 Human)
UseTagsGFPExpressionMammalianMutationT24A S256A S319APromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJFRC5-5XUAS-IVS-mCD8::GFP
Plasmid#26218DepositorInsertmCD8::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-GFP (PX458)
Plasmid#129025Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-huTET1CD, SgRNA cloning site
UseTagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF5-FRT-V5-DEST-GFP-Kif26b
Plasmid#102862PurposeExpresses GFP-Kif26b in mammalian cellsDepositorInsertMouse Kif26b (Kif26b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-H2-Kb SCT-T2A-GFP
Plasmid#205454Purposelentiviral plasmid for expression of mouse H2-Kb SCTDepositorInsertH2-Kb SCT (H2-K1 Mouse)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-mSNCA
Plasmid#185714PurposeAAV expression of GFP and mouse α-Synuclein from hSyn promoterDepositorInsertsynuclein, alpha (Snca Mouse)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-GFP-WT-mCherry-WT (5FP-WW)
Plasmid#189794PurposeExpress GFP/mCherry linked wild-type beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorInsertbeta-globin (HBB Mouse, Human)
UseTagsExpressionMammalianMutationPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only