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Plasmid#65275PurposeGalT reporter only ( no hook) (RUSH system)DepositorInsertgalactosidase
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-puro-mU6-CNPshRNA1
Plasmid#126611PurposeExpresses shRNA against human CNP under mouse U6 promoterDepositorInsertCNP ShRNA-1 (CNP Human)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMuro
Plasmid#82504PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInserteGFP-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSuper.Retro.Puro-WDR5 shRNA 4
Plasmid#59977PurposeKnockdown of WDR5DepositorInsertWDR5 shRNA
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR-puro-RalBP1
Plasmid#31115DepositorInsertRalBP1 shRNA (RALBP1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro3+mRFP-Utr_CH
Plasmid#64358Purposebicistronic expression of Utrophin-mRFP and Puro resistanceDepositorInsertUtrophin-mRFP (UTRN Human)
UseTagsIRES-Puro and mRFPExpressionMammalianMutationQ236R; aa1-261 Utrophin presentPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR-puro-Sec5
Plasmid#31117DepositorInsertSec5 shRNA (EXOC2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pB-puro-mU6-CNPsgRNA
Plasmid#126610PurposeExpresses CNP sgRNA under mouse U6 promoterDepositorInsertCNP sgRNA (Cnp Mouse)
UseCRISPRTagsExpressionMutationPromotermouse U6 promoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/tGFP.HuR.S221D
Plasmid#110351PurposeDoxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and tGFPExpressionMammalianMutationS221D, P237SPromotergagAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-PuroTK-L2
Plasmid#141005PurposeMutisite Gateway mediated vector constructionDepositorInsertPuroTK
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSuper.Retro.Puro-PKN1 shRNA 4
Plasmid#59978PurposeKnockdown of PKN1DepositorInsertPKN1 shRNA
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-cfPar3_4
Plasmid#26706DepositorInsertDog Par3 RNAi
UseLentiviral and RNAiTagsExpressionMammalianMutationDog Par3 RNAiPromoterAvailable sinceJan. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
PSuperPuroRetro-1660s(hPot1)
Plasmid#11136DepositorInsertRNAi against Human Protection of Telomeres 1 (control) (POT1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFIV-H1-Puro-hPolß
Plasmid#18663DepositorInsertshRNA specific to human DNA polymerase beta (POLB Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationnonePromoterAvailable sinceOct. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL3-2
Plasmid#109010PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL3-sgRNA #2 (ATL3 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-1
Plasmid#109008PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL2-sgRNA #1 (ATL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/mKO2
Plasmid#110353PurposeDoxycycline inducible mammalian retroviral expression of mKO2 with FLAG tagDepositorInsertmonomeric Kusabira Orange 2
UseRetroviralTagsFLAGExpressionMammalianMutationPromotergagAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shPIK3CD.v1 puro
Plasmid#58706PurposeLentiviral shRNA vector for knockdown of human PIK3CDDepositorInsertshPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOZ-Pax7-FH-C-puro
Plasmid#32518DepositorInsertPax7d (Pax7 Mouse)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK1 CBM
Plasmid#53196PurposeExpresses HA tagged human MEK1 M187A/H188A/M230A/H239A from puromycin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationM187A/H188A/M230A/H239APromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only