Addgene

We narrowed to 1,309 results for: pEGFP-c1

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mNeongreen-aGBD(anillin)

Plasmid

#129633

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding domain of anillin (712-786)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Tac-GFP

Plasmid

#162494

Purpose

Expresses Tac (IL2 recptor alpha subunit) with C-terminally tagged GFP in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

Tac (IL2RA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus-RnBarr2

Plasmid

#137799

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRcCMV Rootletin (Nigg pFL6(CW501))

Plasmid

#41167

Depositor

Erich Nigg

Article

Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

Insert

Rootletin (CROCC Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pAT9751-BEAR-mCherry-preedited

Plasmid

#162993

Purpose

BEAR control plasmid with split mCherry and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta4

Plasmid

#101292

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

76-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRB

Plasmid

#193568

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Use

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta6

Plasmid

#101294

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

375-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-RnBarr1

Plasmid

#137796

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-GEM

Plasmid

#111191

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

Insert

GEM (GEM Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T2A

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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mNeongreen-pGBD(PKN1 codon optimized)

Plasmid

#129634

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding doamin of PKN1(30 - 100) codon optimized

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTK165_Mem-mCherry-4.1G-CTD

Plasmid

#46362

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G C-terminal domain (EPB41L2 Human)

Use

Tags

Mem (N-terminal 20 amino acids of neuromodulin fo…

Expression

Mammalian

Mutation

C-terminal domain from 886-1005 amino acids

Promoter

CMV

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta7

Plasmid

#101295

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

727-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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N-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45239

Purpose

sensor targeted to N-terminus of Hec1

Depositor

Michael Lampson

Article

Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

Insert

Hec1, Aurora B FRET sensor

Use

Tags

Aurora B FRET sensor and Hec1

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta5

Plasmid

#101293

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

375-727 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-Rac1-G12V-deltaCaaX

Plasmid

#176124

Purpose

Constitutively active, mitochondrial localized Rac1

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RAC1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#79676

Purpose

mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L182A/D183A/I184A/G185A catalytic mutant

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pGFP-Ub-VPS13D_dN

Plasmid

#176465

Purpose

express VPS13D lacking N-terminal 117 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9753-BEAR-mScarlet-preedited

Plasmid

#162994

Purpose

BEAR control plasmid with split mScarlet and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Py2087ACR1

Plasmid

#153032

Purpose

Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

Py2087ACR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV (+ enhancer)

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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