Addgene

We narrowed to 2,255 results for: PRK

Showing: 1001 - 1020 of 2255 results

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pWZLblasti-HA-ERK2 GOF

Plasmid

#53174

Purpose

Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S/D321N

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T3-HA-ERK2 K54R

Plasmid

#53170

Purpose

Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

K54R

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-ERK2 R67S

Plasmid

#53172

Purpose

Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-ERK2 D321N

Plasmid

#53173

Purpose

Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D321N

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pFUGW-shRIIα-RIIαWT-IRES-EGFP

Plasmid

#183456

Purpose

Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag p38 alpha(agf)

Plasmid

#20787

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced Thr180 and Tyr182 with Ala and Phe, resp…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-sgAMPKa1-Cas9-GFP

Plasmid

#208049

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97A-IRES-EGFP

Plasmid

#183457

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97E-IRES-EGFP

Plasmid

#183458

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgAMPKa2

Plasmid

#138685

Purpose

Expresses a human AMPKa2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa2 human (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST p38KTRmCerulean3

Plasmid

#59155

Purpose

Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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MAPK14 gRNA (BRDN0001145809)

Plasmid

#77923

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK14 (MAPK14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK14 gRNA (BRDN0001148417)

Plasmid

#77924

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK14 (MAPK14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LMP AMPK alpha1 hp1

Plasmid

#89489

Purpose

shRNA against AMPK alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Mouse, Human)

Use

Mouse Targeting and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
MAPK14 gRNA (BRDN0001148584)

Plasmid

#77922

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK14 (MAPK14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK14 gRNA (BRDN0001487162)

Plasmid

#77925

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK14 (MAPK14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GRK4 gRNA (BRDN0001145215)

Plasmid

#77723

Purpose

3rd generation lentiviral gRNA plasmid targeting human GRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GRK4 (GRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GRK4 gRNA (BRDN0001146308)

Plasmid

#77724

Purpose

3rd generation lentiviral gRNA plasmid targeting human GRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GRK4 (GRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GRK4 gRNA (BRDN0001145617)

Plasmid

#77725

Purpose

3rd generation lentiviral gRNA plasmid targeting human GRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GRK4 (GRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

Plasmid

#65417

Purpose

AAV packaging vector for hSyn driven KOR DREADD expression.

Depositor

Bryan Roth

Article

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Has Service

AAV8

Insert

hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Expression

Mutation

Promoter

hSyn

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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