Addgene

208,743 results

Showing: 10161 - 10180 of 208743 results

Results per page:

Sort results by:

pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX

Plasmid

#208693

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3h

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-CMV

Plasmid

#72265

Purpose

Express gene of interest under CMV promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

Viral Prep

#61422-LVC

Purpose

Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Promoter

EF1a

Tags

None

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only
AAV-mOXT-hM3Dq-mCherry-WPRE

Plasmid

#70717

Purpose

Expresses DREADDs Gq receptor under oxytocin promoter control

Depositor

Daniel Geschwind

Article

Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

Insert

hM3Dq-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

mouse oxytocin

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL623

Plasmid

#58494

Purpose

helper plasmid for bacterial conjugal DNA transfer

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Insert

encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pML104-HygMx4

Plasmid

#83475

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

HphMX4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pKM496 (deletions) Zeo

Plasmid

#109301

Purpose

ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integrating plasmid

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
htrpC6-YFP

Plasmid

#21084

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

TrpC6 (TRPC6 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Flag-RNF125

Plasmid

#122045

Purpose

express RNF125

Depositor

Ze'ev Ronai

Insert

RNF125 (RNF125 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
V. natriegens NC1

Bacterial Strain

#215355

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Bacterial Resistance

None

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
pET-His-SCov2-nsp8

Plasmid

#154758

Purpose

Expression of SARS-CoV-2 nsp8

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-MRLC1

Plasmid

#35680

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-ETF1-E55D

Plasmid

#130876

Purpose

Encodes ETF1 with an E55D substitution

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

Eukaryotic transcritional termination factor ETF1-E55D (ETF1 Human)

Use

Tags

Expression

Mammalian

Mutation

E55D

Promoter

CAG

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNAZeo(-) LAMP2C

Plasmid

#89342

Purpose

Expresses human LAMP-2C in mammalian cells

Depositor

Janice Blum

Article

Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

Insert

Lysosomal-associated membrane protein 2C (LAMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Use

Tags

GFP-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAM1.19_PB-CIT-PGK-Blast

Plasmid

#205564

Purpose

Express human CIT

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

CIT (CIT Human)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF0688 Kp03_attP-mCherry donor

Plasmid

#193467

Purpose

Promoterless mcherry donor for Kp03

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge