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pEE515

Plasmid

#149288

Purpose

T-DNA encoding TRV2 complete genome with MCS

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pmNFL

Plasmid

#83127

Purpose

Mouse neurofilament protein L cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFL (Nefl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.tgoGAP1

Plasmid

#78737

Purpose

Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

tgoGAP1

Use

Tags

N terminal 81 amino acids of human galactosyltran…

Expression

Mammalian

Mutation

D117A/D119A/D163A in aequorin moiety

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-c-Myc-T58A

Plasmid

#13372

Purpose

Retroviral expression of mouse c-Myc T58A mutant

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (T58A mutant) (Myc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Tyrosine 58 to Alanine

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1 1-150

Plasmid

#24389

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 1-150.

Promoter

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 GFP LIC cloning vector (6D)

Plasmid

#30127

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0

Plasmid

#153288

Purpose

Express the adenosine sensor GRAB_Ado1.0 in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Tags

Expression

Mutation

Promoter

GfaABC1D

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAD-sfGFP(Sp)-Pman

Plasmid

#133833

Purpose

Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

sfGFP(Sp)

Use

E.coli/bacillus spp. shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Pman

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MP4

Plasmid

#69652

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMIEG3-c-Jun

Plasmid

#40348

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

c-Jun (JUN Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pSimple-miR302/367

Plasmid

#98748

Purpose

Episomal reprogramming

Depositor

James Thomson, Sara Howden

Article

Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

Insert

miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLL39

Plasmid

#15458

Depositor

Chia Lee

Article

Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

Insert

Use

S. aureus-e. coli shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 9, 2007

Availability

Academic Institutions and Nonprofits only

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lenti-RfxCas13d-Blast-BFP

Plasmid

#196726

Purpose

Sortable and selectable RfxCas13d.

Depositor

Bernhard Schmierer

Insert

RfxCas13d-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mutation

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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p406ADH1

Plasmid

#15974

Depositor

Nicolas Buchler, Fred Cross

Insert

ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

ADH1 promoter is between SacI and XbaI restrictio…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hFIP200

Plasmid

#24300

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

FIP200 (RB1CC1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-tdTomato

Plasmid

#73462

Purpose

Expresses tdTomato for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

tdTomato

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.Cre

Plasmid

#170367

Purpose

AAV vector expressing Cre under human synapsin promotor

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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mEos3.2-N1

Plasmid

#54525

Purpose

Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Tao Xu, Michael Davidson

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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