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Plasmid#214998PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template
Plasmid#214999PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1050
Plasmid#209540PurposeBTK Type 1 homology arm for Golden Gate assemblyDepositorInsertUpstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1054
Plasmid#209544PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome at a neutral siteDepositorInsertRecombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1061
Plasmid#209550PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA geneDepositorInsertE2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-R334D-TSF
Plasmid#210841PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationR334DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-R326D-TSF
Plasmid#210840PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationR326DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-V314D-TSF
Plasmid#210839PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationV314DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-V44D-TSF
Plasmid#210843PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationV44DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-moxGFP
Plasmid#207744PurposeExpression of the Cre recombinase linked to a moxGFP fluorescent markerDepositorArticleInsertCre-PGK-moxGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL0_62 [sfYFP]
Plasmid#198974PurposeLevel 0 partDepositorInsertsfYFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-paGFP-bPAC-CAAX
Plasmid#204671PurposeMammalian expression of membrane-bound bPAC tagged with paGFP for cAMP productionDepositorArticleInsertpaGFP-bPAC-CAAX
UseLentiviralTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_35 [aadA]
Plasmid#198948PurposeLevel 0 partDepositorInsertaadA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_64 [mScarlet]
Plasmid#198975PurposeLevel 0 partDepositorInsertmScarlet-I
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_57 [PtetL]
Plasmid#198970PurposeLevel 0 partDepositorInsertPtetL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_44 [hph]
Plasmid#198957PurposeLevel 0 partDepositorInserthph
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_33 [cat]
Plasmid#198946PurposeLevel 0 partDepositorInsertcat
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_99 [bsRBS]
Plasmid#199000PurposeLevel 0 partDepositorInsertbsRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_43 [tsr]
Plasmid#198956PurposeLevel 0 partDepositorInserttsr
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_78 [ORI_BC_3]
Plasmid#198979PurposeLevel 0 partDepositorInsertORI_BC_3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only