Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 981 - 1000 of 1204 results

  9. pcDNA3-ICUE4

    Plasmid
    #181846
    Purpose
    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    ICUE4 (RAPGEF3 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    181846-plasmid-map-sequence-id-364236

    Enlarge

  12. HL 6692

    Bacterial Strain
    #69776
    Purpose
    MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + ΔOxyR
    Depositor
    Han Lim
    Article
    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw
    Bacterial Resistance
    Chloramphenicol
    Availability
    Academic Institutions and Nonprofits only

  13. HL 6693

    Bacterial Strain
    #69777
    Purpose
    MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + Δdam
    Depositor
    Han Lim
    Article
    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw
    Bacterial Resistance
    Chloramphenicol
    Availability
    Academic Institutions and Nonprofits only

  14. HL 6730

    Bacterial Strain
    #69779
    Purpose
    MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + ΔOxyR + Δdam
    Depositor
    Han Lim
    Article
    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw
    Bacterial Resistance
    Chloramphenicol
    Availability
    Academic Institutions and Nonprofits only

  16. Cer(1-158)-gamma-1 in pcDNAI/Amp

    Plasmid
    #55623
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    Cer(1-158)-gamma-1 (GNGT1 Bovine, Aequorea victoria)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-1 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55623-plasmid-map-sequence-id-115166

    Enlarge

  17. Cer(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55624
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    Cer(1-158)-gamma-2 (Gng2 Aequorea victoria, Mouse)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-2 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55624-plasmid-map-sequence-id-115167

    Enlarge

  18. Cer(1-158)-gamma-5 in pcDNAI/Amp

    Plasmid
    #55625
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    Cer(1-158)-gamma-5 (GNG5 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-5 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55625-plasmid-map-sequence-id-115180

    Enlarge

  19. Cer(1-158)-gamma-7 in pcDNAI/Amp

    Plasmid
    #55626
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    Cer(1-158)-gamma-7 (GNG7 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-7 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55626-plasmid-map-sequence-id-115182

    Enlarge

  20. Cer(1-158)-gamma-10 in pcDNAI/Amp

    Plasmid
    #55627
    Purpose
    An N-terminal mCerulean fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    Cer(1-158)-gamma-10 (GNG10 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-10 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55627-plasmid-map-sequence-id-115184

    Enlarge

Showing: 981 - 1000 of 1204 results