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Plasmid#101643PurposeEncodes the fluorescent protein meffCFP for constitutive expression in E. coli.DepositorInsertmeffCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta11 Aphrodite
Plasmid#13569DepositorInsertFLIPglu F16A Delta11 Aphrodite
UseTagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSM14
Plasmid#101645PurposeEncodes the fluorescent protein efasCFP for constitutive expression in E. coli.DepositorInsertefasCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta13 Aphrodite
Plasmid#13570DepositorInsertFLIPglu F16A Delta13 Aphrodite
UseTagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-Sec22b
Plasmid#168029PurposeExpresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cellsDepositorInserteCFP-Sec22b(A131-H190)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-VAMP8
Plasmid#168028PurposeExpresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cellsDepositorInserteCFP-VAMP8(G7-R67)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSM13
Plasmid#101644PurposeEncodes the fluorescent protein meleCFP for constitutive expression in E. coli.DepositorInsertmeleCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
QUAS-jGCaMP7s-T2A-tdTomato
Plasmid#200010PurposeQUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2ADepositorInsert3xP3-ECFP-jGCaMP7s-td-Tomato
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CR
Plasmid#171461PurposeExpresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cellsDepositorInsertTom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL204
Plasmid#22282PurposeExpresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membraneDepositorInsertpCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic Fyn FRET Biosensor
Plasmid#124654PurposeFRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPetDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL113
Plasmid#22471PurposeExpresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.DepositorInsertPhyB NT (PHYB Mouse, Mustard Weed)
UseSynthetic BiologyTags10aaLinker-mCFP-HrasCTExpressionMammalianMutationY276HPromoterCMVAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-InPAkt
Plasmid#181915PurposeIndicator of Phosphoinositides using Akt; targeted to the plasma membrane.DepositorInsertpm-InPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationContains the following mutations with respect to …PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBullet-er-c
Plasmid#53068Purposedestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-c
Plasmid#53066Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A40
Plasmid#131994PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A40 (SLC25A40 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
CF-AIP
Plasmid#61526PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with ECFP and AIPDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP and ECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-n
Plasmid#53065Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only