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PurposeExpresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5369
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT
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Alt namephyB
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)3551
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Entrez GenePHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Kras4B [Entrez Gene ID: 3845] polybasic CaaX terminus
(KKKKKKSKTKCVIM) [ggt aaa aag aag aaa aag aag tca aag aca aag tgt gta att atg].
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAL204 was a gift from Christopher Voigt (Addgene plasmid # 22282 ; http://n2t.net/addgene:22282 ; RRID:Addgene_22282) -
For your References section:
Spatiotemporal control of cell signalling using a light-switchable protein interaction. Levskaya A, Weiner OD, Lim WA, Voigt CA. Nature. 2009 Oct 15;461(7266):997-1001. 10.1038/nature08446 PubMed 19749742