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Plasmid#129651Purpose2P-activatable CRY2DepositorInsert2paCRY2
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9
Plasmid#78903PurposeExpresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertdCas9
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCk2_spacer
Plasmid#136072PurposeUse when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCk1_spacer
Plasmid#136071PurposeUse when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACM4
Plasmid#49797PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC574
Plasmid#127975PurposeAMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInserthygB (hygromycin resistance marker)
UseFungal expressionTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
CasRX_Pac
Plasmid#194803PurposeCasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.DepositorInsertCasRX
UseTagsExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2
Plasmid#11343DepositorTypeEmpty backboneUseTagsGal4 Activation DomainExpressionYeastMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC37
Plasmid#111618PurposeFluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosaDepositorInsertPpqsA-gfp(ASV)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2gtwy
Plasmid#11346DepositorInsertgateway cassette
UseTagsGal4 Activation DomainExpressionYeastMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
HypaCas9
Plasmid#138557PurposeExpresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSDDepositorInsertHypaCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN692A, M694A, Q695A, H698APromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACU
Plasmid#58373PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC1750
Plasmid#127984PurposeAMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYC.GFP1Amb
Plasmid#102792PurposeExpresses GFP with a premature stop codonDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterialMutationY39StopPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_PACSIN2-1/1
Plasmid#91330PurposeProtein expression and purification of human SH3 domain construct PACSIN2-1/1DepositorInsertPACSIN2-1/1 (PACSIN2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC572
Plasmid#127973PurposeAMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertpyrG
UseFungal expressionTagsExpressionBacterialMutationPromoternative promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only