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Plasmid#210426PurposeBacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage siteDepositorInsertp62 FL dUBA (1-389) (NUP62 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-TrxF
Plasmid#207454PurposeE. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tagDepositorInsertThioredoxin F
UseTags6xHisExpressionBacterialMutationCodon optimized for E coli expressionPromoterT7Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPTM p62 PB1 (1-122)
Plasmid#210428PurposeBacterial expression of MBP-PB1(1-122) with TEV cleavage siteDepositorInsertp62 PB1 (1-122) (NUP62 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM493
Plasmid#140191PurposeORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.DepositorInsertTEV-Flag-Gly4-eGFP
UseTagsFlag + eGFPExpressionBacterialMutationPromoterPGroELAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL2108
Plasmid#201969PurposeORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.DepositorInsertattB-3C-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 3MYC SMASh
Plasmid#182524PurposeC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).DepositorInsertSMASh Tag
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 3MYC SMASh
Plasmid#182525PurposeC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).DepositorInsertSMASh Tag
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-GAI
Plasmid#183405PurposeExpresses nuclear-localized LgBiT-GAIDepositorInsertLgBiT-GAI
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-gfp-Kir2.1-HA-tevs-KKYL
Plasmid#179706PurposeTEVP-inducible GFP-conjugated Kir2.1 RELEASE with KKXX motifDepositorInsertKir2.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRMCD111
Plasmid#172957PurposepRMCD77 encoding Wzz mutant G311ADepositorInsertShigella flexneri wzz G311A mutant gene
UseTagsExpressionBacterialMutationD168G and N33D- please see depositor commentPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCD113
Plasmid#172959PurposepRMCD77 encoding Wzz mutant G305A/G311ADepositorInsertShigella flexneri wzz G305A/G311A mutant gene
UseTagsExpressionBacterialMutationG305A/G311A, A208V - please see depositor commentsPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crG18
Plasmid#172930PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSGDepositorInsertCrowdingSensorG18
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0549
Plasmid#162300PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, SUMO-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0550
Plasmid#162301PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-SUMO-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPMY0SP0002
Plasmid#162304PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, 6xHis tag-[HRV 3C cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0551
Plasmid#162303PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only