pRMCD113
(Plasmid
#172959)
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PurposepRMCD77 encoding Wzz mutant G305A/G311A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRMCD77
- Backbone size w/o insert (bp) 3300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameShigella flexneri wzz G305A/G311A mutant gene
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SpeciesShigella flexneri
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Insert Size (bp)1165
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MutationG305A/G311A, A208V - please see depositor comments
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor confirms additional A208V mutation does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRMCD113 was a gift from Renato Morona (Addgene plasmid # 172959 ; http://n2t.net/addgene:172959 ; RRID:Addgene_172959) -
For your References section:
Analysis of Shigella flexneri wzz (Rol) function by mutagenesis and cross-linking: wzz is able to oligomerize. Daniels C, Morona R. Mol Microbiol. 1999 Oct;34(1):181-94. doi: 10.1046/j.1365-2958.1999.01591.x. 10.1046/j.1365-2958.1999.01591.x PubMed 10540296