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Plasmid#166759PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BidDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of mouse Bid …PromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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Venus-BimEL(Bad)-pEGFP-C1
Plasmid#166758PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BadDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Bad …PromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-GAPDH-IRES-EGFP
Plasmid#115918Purposedonor to insert IRES-EGFP at the GAPDH locus in human cellsDepositorInsertIRES-EGFP
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP-mirMecp2
Plasmid#163704PurposepAAV plasmid to induce expression of micro-RNA targeted against mouse Mecp2 and EGFPDepositorInsertmirMecp2 and EGFP (Mecp2 Aequorea victoria, Mouse)
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP-mirNega
Plasmid#163705PurposepAAV plasmid to induce expression of universal negative control micro-RNA and EGFPDepositorInsertmirNega and EGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KAR2-mEGFP-HDEL
Plasmid#160467PurposeYeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signalDepositorInsertKAR2-mEGFP-HDEL
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::T48-Baz::vhhGFP4
Plasmid#163928PurposeExpression of GrabFP-A-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GSX2-P2A-tGFP-DTA
Plasmid#161750PurposetGFP plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertturboGFP
UseCRISPRTagsP2A-tGFPExpressionMammalianMutationPromoterendogenous GSX2 promoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
hPGK-ABE7.10-P2A-GFP
Plasmid#162511PurposeExpress ABE7.10-P2A-GFP by hPGK promoter in mammalian cellsDepositorInsertABE7.10-P2A-GFP
UseCRISPRTagsNLSExpressionMammalianMutationPromoterhPGKAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EFS-ABE7.10-P2A-GFP
Plasmid#162510PurposeExpress ABE7.10-P2A-GFP by EFS promoter in mammalian cellsDepositorInsertABE7.10-P2A-GFP
UseCRISPRTagsNLSExpressionMammalianMutationPromoterEFSAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 (184-618)
Plasmid#127626PurposeHuman Miro1 lacking the first GTPase domainDepositorInsertMiro1 (RHOT1 Human)
UseTagseGFPExpressionMammalianMutation184-618 onlyPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 (412-618)
Plasmid#127628PurposeGTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acidsDepositorInsertMiro1 (RHOT1 Human)
UseTagseGFPExpressionMammalianMutation412-618 onlyPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP
Plasmid#154368PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 ΔGRM-ΔC
Plasmid#112659PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 ΔGRM ΔC
UseTagssfGFPExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 A
Plasmid#112660PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 S389,398A
UseTagssfGFPExpressionYeastMutationmutations (S389A, S398A) in Glo3 motif, non-phosp…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
UseTagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 Δ2x+ve
Plasmid#112662PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 Δ2x+ve
UseTagssfGFPExpressionYeastMutationMutations (R223E, K224E, K225E, K233E, K234E, K23…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dox nontargeting shRNA GFP
Plasmid#155290PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-QUASM1:GFPNLS-SV40pA
Plasmid#155116PurposeMiddle entry vector containing QUASM1 element upstream of GFP-NLS.DepositorInsertQUASM1:GFPNLS
UseGateway middle entry vectorTagsExpressionMutationPromoterQUASM1-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only