Addgene

We narrowed to 2,255 results for: PRK

Showing: 961 - 980 of 2255 results

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pcDNA3-RIIα-μDpTT

Plasmid

#182327

Purpose

PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)

Use

Tags

Full-length human PKA type 2alpha regulatory subu…

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC011

Plasmid

#171143

Purpose

Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Sp.pCas9, BBa_J23107

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-shp38

Plasmid

#52920

Purpose

expresses a hairpin specific for human p38MAPK

Depositor

Sheila Stewart

Article

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting mitogen-activated protein kinase p38 (MAPK14 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-Nix

Plasmid

#100770

Purpose

Lentiviral expression of shRNA targeting Nix

Depositor

Joseph Gordon

Article

da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

Insert

Lenti-sh-Nix

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN Parkin

Plasmid

#89299

Purpose

For expression of untagged Parkin

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 v2 neo

Plasmid

#72570

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 puro

Plasmid

#72567

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 v2 puro

Plasmid

#72568

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MKK7a1-EE

Plasmid

#47578

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK7a1-EE (Map2k7 Mouse)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Residues 73-420 of RefSeq sequence. Ser271 to Gl…

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pGSTag Flag p38 alpha (agf)

Plasmid

#20816

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pGSTag Flag p38 alpha (agf) (Mapk14 Mouse)

Use

Tags

Flag

Expression

Bacterial

Mutation

Replace Thr 180 and Tyr 182 with Ala and Phe resp…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
shMEK1/2 neo

Plasmid

#72569

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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3xFlag-tdMCP-Erk2 (pErk reporter)

Plasmid

#226167

Purpose

Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

3xFlag-tdMCP-Erk2 (MAPK1 Human)

Use

Tags

3xFlag-tdMCP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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PCDNA3 Flag p38 alpha (agf)

Plasmid

#20352

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Replace Thr 180 and Tyr 182 with Ala and Phe resp…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pBMN BFP-Parkin

Plasmid

#186221

Purpose

For expression of Parkin tagged with BFP

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-mKOR

Plasmid

#179285

Purpose

Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

Insert

kappa opioid receptor (Oprk1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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PKN1 gRNA (BRDN0001162558)

Plasmid

#76278

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN1 (PKN1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MAPK14-P2A-Hygro_Barcode

Plasmid

#170239

Purpose

Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK14 (MAPK14 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only