Showing: 9701 - 9720 of 14149 results
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Plasmid#98231PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
KLHL40 (KBTBD5A-c006)
Plasmid#98233PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKBTBD5A (KLHL40 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
RECQL5 (RECQL5A-c104)
Plasmid#98234PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
IPCEF1 (PIP3EA-c002)
Plasmid#98240PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPIP3EA (IPCEF1 Human)
UseTagsHis-6-TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
BRD7 (BRD7A-c005)
Plasmid#98245PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRD7A (BRD7 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00439:Bromodomain 136-223PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pNCS Citrine
Plasmid#91761PurposeBacterial expression of a fluorescent protein, CitrineDepositorInsertCitrine
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCX-H2B-KikGR
Plasmid#98649PurposeExpresses a nuclear-localized photoconvertible green-to-red fluorescent proteinDepositorInsertKikGR
UseTagsH2BExpressionMammalianMutationPromoterCMV-IE enhancer chick beta actin promoterAvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-LaminB
Plasmid#98830PurposeIn vivo visualization of the nuclear envelope (can be used for colocalization studies)DepositorArticleInsertLaminB
UseTagsmTurquoise2ExpressionMammalianMutation*(see below)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mScarlet-I-LaminB
Plasmid#98831PurposeIn vivo visualization of the nuclear envelope (can be used for colocalization studies)DepositorArticleInsertCytERM
UseTagsmScarlet-IExpressionMammalianMutation*(see below)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
14UAS zfPSD95:GFP
Plasmid#74314PurposeA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatsDepositorInsertzebrafish PSD95
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterUASAvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP
Plasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
UseTagsEGFPExpressionMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailabilityAcademic Institutions and Nonprofits only -
pSL690
Plasmid#47753PurposeExpresses dCas9-VP64 fusionDepositorInsertdCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6AvailabilityAcademic Institutions and Nonprofits only -
pAGM1467
Plasmid#50298DepositorInsert5'UTR, omega (Tobacco Mosaic Virus) + HIS tag + enterokinase cleavage site
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICSL50016
Plasmid#50315DepositorInsertCDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
UsePlant expressionTagsExpressionMutationCodon-optimised for A. thaliana. BsaI/BbsI sites …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pICSL50015
Plasmid#50318PurposePlease note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)DepositorInsertCDS, mNEON (Branchistoma lanceolatum)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-L2-TALEN
Plasmid#51554PurposeExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.DepositorInsertH11-L2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 9701 - 9720 of 14149 results