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pgRNA

Plasmid

#215560

Purpose

Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

gRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDRM166 LKB (Luciferase mKate2 Blast)

Plasmid

#183502

Purpose

Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
mKate2
BSD

Use

Lentiviral and Luciferase

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Mutation

Promoter

MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn FAK biosensor

Plasmid

#78299

Purpose

FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Lyn Fak biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLPC hRap1-FL

Plasmid

#12542

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 (TERF2IP Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pBMN(CMV-copGFP-Puro-SMAR)

Plasmid

#80391

Purpose

expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

Depositor

Magnus Essand

Article

Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

Insert

copGFP-T2A-Puromycine

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTCF_WT

Plasmid

#81789

Purpose

Gateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTCF (CTCF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217342

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDAS12069_U6-pegRNA-mCherry

Plasmid

#177180

Purpose

pegRNA cloning plasmid with mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPalmitoyl-mTurquoise2

Plasmid

#36209

Purpose

In vivo visualization of the membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

p63(1-29) (ARHGEF25 Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX58-ts3

Plasmid

#174243

Purpose

DDX58 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX58 shRNA (RIGI Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mPCSK9)

Plasmid

#170122

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Pcsk9 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pmVenus(L68V)-mTurquoise2

Plasmid

#60493

Purpose

Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

SYFP2-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-NG

Plasmid

#159977

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-NG

Use

Tags

BpSV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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mScarlet-Rab7

Plasmid

#169068

Purpose

Express human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_NoFlag_hROSA26_Left

Plasmid

#191442

Purpose

Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

Depositor

Yannick Doyon

Insert

hROSA26 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pemiRFP670-tubulin

Plasmid

#136573

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP670-tubulin

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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miniTurbo-His6_pET21a

Plasmid

#107178

Purpose

expresses His6-tagged miniTurbo in bacteria

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Tags

Expression

Bacterial

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDR119

Plasmid

#13332

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

DNA ligase IV (LIG4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
AAV-CAG-ChR2-GFP

Plasmid

#26929

Purpose

AAV-mediated expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pET15b_Bst-LF

Plasmid

#153313

Purpose

Bacterial expression of the Bst Large Fragment

Depositor

Drew Endy, Philippa Marrack

Insert

Bst Large Fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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