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Plasmid#189676Purposetitratable expression of sfGFP in bacteriaDepositorInsertsfGFP
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD86-mEYFP(Q69K)-mEGFP
Plasmid#190750PurposeExpression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmEGFP and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IDO2-p2a-eGFP
Plasmid#187027PurposeExpresses IDO2 and GFP (seperately) in mammalian cellsDepositorInsertIndoleamine 2,3-dioxygenase 2 (IDO2 Human)
UseTagsP2A-GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR/Zeo-sfGFP-SKL
Plasmid#186720PurposeGateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame).DepositorInsertsfGFP-SKL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mCherry2-mEGFP
Plasmid#182859PurposeHetero-dimer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mCherry2-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-GFP_mmu-miR-16-5p_sponge
Plasmid#132624PurposeExpresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTRDepositorInsertstop codon, mmu-miR-16-5p sponge sequence and polyA signal
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-Alox5-PLAT-EGFP-6H
Plasmid#187189PurposeProtein ExpressionDepositorInsertENSDARG00000057273 (alox5a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP1-Y34A_P
Plasmid#147214PurposeInsect Expression of DmDCP1-Y34ADepositorInsertDmDCP1-Y34A (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP2-PC-V5His6_G
Plasmid#146348PurposeInsect Expression of DmDCP2-PCDepositorInsertDmDCP2-PC (DCP2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP1_327-372_H
Plasmid#146500PurposeInsect Expression of DmDCP1_327-372DepositorInsertDmDCP1_327-372 (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15 PemrR-gfp biosensor
Plasmid#185793PurposeThe pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.DepositorInsertpEmrR-gfp
UseTagsEGFPExpressionBacterialMutationPromoterPemrRAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Plekhh1-MyTH-FERM
Plasmid#187370PurposeExpresses human Plekhh1-MyTH-FERM labelled with EGFPDepositorInsertPlekhh1-MyTH-FERM
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-Alox12-PLAT-EGFP-6H
Plasmid#187190PurposeProtein ExpressionDepositorInsertENSDARG00000069463 (alox12 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-His-sfGFP
Plasmid#180754PurposepET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationG140C- Please see depositor commentsPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-UBC-EGFP-2A-dnVAMP2
Plasmid#185723PurposeLentiviral expression of GFP and dominant-negative VAMP2 from UbC promoterDepositorInsertVAMP-2 (VAMP2 Human)
UseLentiviralTagsExpressionMammalianMutationdeleted AA 97-116PromoterhUbCAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG8H-GFP-EWS_1-120
Plasmid#180464PurposeExpresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120DepositorInsertEWSR1 (EWSR1 Human)
UseTagsGFP and His8ExpressionBacterialMutationdeleted 121f-656PromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFOX12-Tier1-PhCMV-eGFP
Plasmid#169509PurposeTier-1 vector encoding PhCMV-driven green fluorescent protein eGFP (PhCMV-eGFP-pA).DepositorInsertPCMV-driven enhanced green fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VP16
Plasmid#183935PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VP16 (UL48 Synthetic)
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8133 MSCV GFP Neo C-HA
Plasmid#184522PurposeExpression of GFPDepositorInsertGFP
UseRetroviralTagsHAExpressionMutationPromoterMSCV LTRAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf cdh1-deltaCyot-GFP
Plasmid#185412PurposeGFP tagged zebrafish cdh1 lacking the cytoplasmic domain.DepositorInsertcdh1 (cdh1 Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationCytoplasmic domain deletedPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only