1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pLenti_EF1a_TauFRET_3xKQ_wpre

   Plasmid

   #207522

   Purpose

   Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

   Depositor

   Bradley Hyman

   Article

   Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

   Insert

   TauFRET_P301L_3xKQ
   

   Use

   Lentiviral

   Tags

   NeonGreen and mTurquoise2

   Expression

   Mutation

   P301L; K311Q; K317Q; K321Q

   Promoter

   EF1a

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH75

   Plasmid

   #128224

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. modified pcAT7-Glo1

   Plasmid

   #160996

   Purpose

   Splicing reporter backbone

   Depositor

   Brenton Graveley

   Article

   Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

   Insert

   hemoglobin subunit beta (HBB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Repeated intron 1 with 40 nucelotide deletion

   Promoter

   CAG

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a(+)::NL

   Plasmid

   #141289

   Purpose

   NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF463

   Plasmid

   #167671

   Purpose

   Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GAL-Aur1-2GFP-Hygromycin

   Plasmid

   #184775

   Purpose

   Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   proGAL-Aur1 (AUR1 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACBSR-hyg

   Plasmid

   #87830

   Purpose

   Expresses lambda-red system with hyg selection marker

   Depositor

   Pep Charusanti

   Article

   Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

   Insert

   hyg
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   None

   Promoter

   bla

   Available since

   Aug. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry_gRNA

    Plasmid

    #80457

    Purpose

    gRNA expression vector with mCherry transfection control

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Inserts

    sp Cas9 gRNA
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin promoter

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMo130-TelR

    Plasmid

    #50799

    Purpose

    Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

    Depositor

    Kim Lee Chua

    Article

    Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

    Insert

    tellurite-resistance cassette
    

    Use

    Suicide vector

    Tags

    Expression

    Mutation

    Promoter

    PCs12

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEP207-AAV-CMV-MCS-pA

    Plasmid

    #78318

    Purpose

    AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-TLR6

    Plasmid

    #118997

    Purpose

    Targeting vector for human AAVS1 with TLR6 reporter insert

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. BB3rN_14*

    Plasmid

    #98550

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. StrepII-tag

    Plasmid

    #55181

    Purpose

    Streptactin-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    StrepII tag
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p426-SNR52p-gRNA.csr-1.Y-SUP4t

    Plasmid

    #68060

    Purpose

    gRNA for csr-1 locus in N. crassa

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for csr-1 locus
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pI3-MTU-DO-G418

    Plasmid

    #160023

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Use

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFREE_Amp

    Plasmid

    #92051

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. puc19-pCas13a

    Plasmid

    #118963

    Purpose

    Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

    Depositor

    Magdy Mahfouz

    Article

    Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

    Insert

    Cas13a
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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