LeGO-lnc-MONC
(Plasmid
#86966)
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PurposeExpresses lncRNA MONC (Alias: MIR99AHG; LINC00478)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneLEGO-lnc
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin ; Cerulean
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMONC isoform 6
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Alt nameMIR99AHG
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Alt nameLINC00478
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SpeciesH. sapiens (human)
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Entrez GeneMIR99AHG (a.k.a. C21orf34, C21orf35, LINC00478, MONC)
- Promoter SFFV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer SFFV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LeGO-lnc-MONC was a gift from Jan-Henning Klusmann (Addgene plasmid # 86966 ; http://n2t.net/addgene:86966 ; RRID:Addgene_86966) -
For your References section:
LincRNAs MONC and MIR100HG act as oncogenes in acute megakaryoblastic leukemia. Emmrich S, Streltsov A, Schmidt F, Thangapandi VR, Reinhardt D, Klusmann JH. Mol Cancer. 2014 Jul 15;13:171. doi: 10.1186/1476-4598-13-171. 10.1186/1476-4598-13-171 PubMed 25027842
Map uploaded by the depositor.
