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Plasmid#177422PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-2-Cb5, Bcl2-cb5, Bcl-cb5
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-L61G-pEGFP-C1
Plasmid#177426PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationLeucine 61 replaced with glycinePromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-GG153AA-pEGFP-C1
Plasmid#177427PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationGlycines 153 and 154 mutated to alaninePromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-G154STOP-pEGFP-C1
Plasmid#177428PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationGlycine 154 replaced with STOP codonPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3C DD 616-796
Plasmid#193937PurposeMammalian expression of GFP-tagged Kif 3C DD 616-796DepositorInsertKif 3C (Kif3c Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B CC 391-592
Plasmid#193930PurposeMammalian expression of GFP-tagged Kif 3B CC 391-592DepositorInsertKif 3B (Kif3b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3C CC 411-615
Plasmid#193935PurposeMammalian expression of GFP-tagged Kif 3C CC 411-615DepositorInsertKif 3C (Kif3c Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3A DD 590-702
Plasmid#193927PurposeMammalian expression of GFP-tagged Kif 3A DD 590-702DepositorInsertKif 3A (KIF3A Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3A CC 419-589
Plasmid#193925PurposeMammalian expression of GFP-tagged Kif 3A CC 419-589DepositorInsertKif 3A (KIF3A Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-p3E-GLRX-roGFP2
Plasmid#194288PurposeMultisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQFDepositorInsertGLRX-roGFP2
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
UseTags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47-Noa1-GFP
Plasmid#191968PurposeGateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning TechnologyDepositorInsertNoa1-GFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-GFP::NMN-Deamidase
Plasmid#187868PurposeGal4/UAS expression of GFP::NMN-DeamidaseDepositorInsertNMN-Deamidase
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #2 GFP
Plasmid#106951PurposeLentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.DepositorInsertgRNA targeting Notch1 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #3 GFP
Plasmid#106952PurposeLentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.DepositorInsertgRNA targeting Notch1 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Hic1 GFP
Plasmid#106953PurposeLentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.DepositorInsertanti-Hic1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)
Plasmid#190134Purposeexpresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cellsDepositorInserteGFP-p2A-HA-GPR151(p.Arg95Ter)
UseLentiviralTagsHA, eGFP, and p2AExpressionMammalianMutationp.Arg95Ter mutationPromoterCMVAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpO
Plasmid#192382PurposeTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when heat shocked.DepositorInsertAct2::B3RT-OCS-B3RT::Act2::FRT-OCS-FRT::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1
Plasmid#192470PurposeTransient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS8
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5 promoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1
Plasmid#192469PurposeTransient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS3
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5 promoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only