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Plasmid#8311DepositorInsertcIAP1 (BIRC2 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 CAG rtTA3 TauWT 2N4R-EGFP
Plasmid#132389PurposeExchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 siteDepositorInserthuman TauWT 2N4R-EGFP (MAPT Human, Synthetic)
UseCRISPR and Cre/LoxTagsEGFPExpressionMammalianMutationPromoterTREtightAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131B
Plasmid#69281PurposeGolden Gate entry vector; 1st gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
openCIDAR MoClo Toolkit
Plasmid Kit#1000000227PurposeThis collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.DepositorAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS-WT
Plasmid#44978DepositorInsertFUS (Full length) (FUS Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-dTomato
Plasmid#99376PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-dTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNestin-EGFP
Plasmid#38777DepositorInsertnestin 2nd intron enhancer with TK mini promoter (NES Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)3
Plasmid#126955PurposeBacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagseGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-CMV-hErk1
Plasmid#49328PurposeExpresses FLAG-tagged human Erk1DepositorInsertErk1 (MAPK3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pDONRp5-p2 GFP-NShroom3-iLID
Plasmid#170976PurposeN-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.DepositorInsertNShroom3 (Shroom3 Mouse)
UseGateway cloningTagseGFP and iLIDExpressionMutationFrom isoform 2, deleted amino acids 1389 - 1805,…PromoterAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 SspB-mCherry-CShroom3
Plasmid#170977PurposeC-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.DepositorInsertCShroom3 (Shroom3 Mouse)
UseGateway cloningTagsSspB and mCherryExpressionMutationIsoform X2, deleted aminoacids 1 - 1388PromoterAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-FRT hM4Di-mCherry
Plasmid#161576PurposeExpression of hM4Di-mCherry in a Flp-dependent fashionDepositorInserthM4Di-mCherry
UseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFR-miRFP670nano3
Plasmid#184673PurposeEGF receptor (EGFR) labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertEGFR-miRFP670nano3
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorInsertssSpCas9
gRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
BRAF in pENTR
Plasmid#216605Purposegateway cloning donor vector containing BRAFDepositorInsertBRAF (BRAF Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-hMCP-mScarlet-I-NLS
Plasmid#174878PurposePiggyBac transposon vector for expression of MCP-mScarlet-I-NLSDepositorInsertMCP-mScarlet-I-NLS
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-3xFLAG-V5-ccdB
Plasmid#87072PurposeLentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseLentiviralTags3xFLAG-V5ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b
Plasmid#122187PurposeBacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV2)
Viral Prep#50477-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only