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Addgene

pLenti6.2-3xFLAG-V5-ccdB
(Plasmid #87072)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87072 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti6.2
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin
  • Tag / Fusion Protein
    • 3xFLAG-V5 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • 3xFLAG-V5 (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.2-3xFLAG-V5-ccdB was a gift from Susan Lindquist & Mikko Taipale (Addgene plasmid # 87072 ; http://n2t.net/addgene:87072 ; RRID:Addgene_87072)
  • For your References section:

    A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways. Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, Choi H, Berger B, Gingras AC, Lindquist S. Cell. 2014 Jul 17;158(2):434-48. doi: 10.1016/j.cell.2014.05.039. 10.1016/j.cell.2014.05.039 PubMed 25036637