-
Plasmid#200899PurposeExpresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.DepositorInsertPseCas7, PseCas8, PseCas6, PseTniQ
UseTagsNLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB1 (CK2alpha)
Plasmid#27083DepositorInsertCK2alpha (CSNK2A1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-Keratin-17
Plasmid#56465PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 488, Emission: 507DepositorInsertKeratin (KRT18 Human)
UseTagsmEGFPExpressionMammalianMutationV282LPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70
Plasmid#62914PurposeCreating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-p65AD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAF0474 Ef1a-Si74_attB acceptor
Plasmid#193475PurposeAcceptor plasmid for 3 plasmid recombination assayDepositorInsertSi74_attB
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-mU6-evopreQ1
Plasmid#210190PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-iRFP670 BlastR
Plasmid#84385PurposeLentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsiRFP670ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2538
Plasmid#144036PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX4 (SOX4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9_GFP
Plasmid#64709PurposeCo-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cellsDepositorInsertSaCas9-2A-GFP
UseCRISPRTags2A-GFP and 3xFLAG-SV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast
Plasmid#205551PurposeExpress EPN24-MCP-I-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP-I
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
UseTagsEGFPExpressionMutationPromoter5UASAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-GFP-α-Tubulin
Plasmid#213900PurposeImaging of Tubulin using Photoactivatable GFPDepositorInsertPA-GFP-α-Tubulin
UseTagsPhotoactivatable GFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMC00022 (aka pRC0433)
Plasmid#195321PurposeLentiviral expression vector derived from LEGO-G2 with hygromycin selectionDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorUseTags6xHis-TEVExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2_ubi:EGFP
Plasmid#27323DepositorInsertubiquitin:EGFP reporter
UseTol2 transposonTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2h
Plasmid#140554PurposeExpresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2h (high affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.1 (AAV1)
Viral Prep#111066-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-dLight1.1 (#111066). In addition to the viral particles, you will also receive purified pAAV-hSyn-dLight1.1 plasmid DNA. hSyn-driven expression of dLight1.1 dopamine sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#191796Purposevector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoterDepositorInserthumanized dCas13X
UseCRISPRTagsExpressionMammalianMutationR84A, H89A, R739A, R740A, H745A, H746A, H747APromoterCBh, SV40, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only