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Addgene

pLentiCRISPR-E
(Plasmid #78852)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78852 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLentiCRISPRV2
  • Backbone size (bp) 14849
  • Modifications to backbone
    espcas9: K848A, K1003A, K1060A
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • Cas9-P2A-Puro

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiCRISPR-E was a gift from Phillip Abbosh (Addgene plasmid # 78852 ; http://n2t.net/addgene:78852 ; RRID:Addgene_78852)
  • For your References section:

    Differential effects of clinically-relevant N- vs C-terminal truncating CDKN1A mutations on cisplatin sensitivity in bladder cancer. Sikder RK, Ellithi M, Uzzo RN, Weader D, Metz AL, Behbahani A, McKenzie ER, El-Deiry WS, Abbosh PH. Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 10.1158/1541-7786.MCR-19-1200. 10.1158/1541-7786.MCR-19-1200 PubMed 33272936