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Plasmid#69928PurposeExpresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.DepositorInsertPllacO1-cisRAJ31-GFP-LAA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlLacO1Available sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
circRAJ31-Toehold_mutant
Plasmid#69930PurposeExpresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.DepositorInsertplTet-circRAJ31_toeholdmutant
UseSynthetic BiologyTagsExpressionMutation4 bases mutated in toehold of riboregulatorPromoterPltetO1-2Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM0617
Plasmid#128993PurposeloxP-KIURA3-loxP preassembled entry vectorDepositorInsertKanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ159
Plasmid#69308PurposeGateway entry vector with hSpCas9D10ADepositorInserthSpCas9D10A (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Integrin-β6 I domain I270C
Plasmid#159639PurposeContains integrin beta 6 residues 108–352 with I270C mutationDepositorInsertIntegrin beta 6 I domain I270C
UseTags6x HisExpressionMammalianMutationI270CPromoterAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-GFP-2xmiR-34 (pAW-AS-25)
Plasmid#40833PurposemiRNA sensor used in Drosophila cell linesDepositorInsertTwo target sites perfectly complementary to miR-34
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB6581
Plasmid#106137PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6580
Plasmid#106136PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6575
Plasmid#106133PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT303_SNR52_sgRNA_Can1-3
Plasmid#145066PurposeExpresses sgRNA targeting Can1-3 siteDepositorInsertCan1-3 sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS Wnt-3HA
Plasmid#17373DepositorInsertWnt-3HA (WNT3 Human, Mouse)
UseTagsHAExpressionBacterialMutationThis gene is a fusion of the murine and human wnt…PromoterAvailable sinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck
Plasmid#58522PurposeExpresses sdChR(C138S) fused to GCaMP6f in mammalian cellsDepositorInsertsdChR(C138S)-TS-GCaMP6f-ER
UseLentiviralTagsGCaMP6fExpressionMammalianMutationchanged Cysteine 138 to SerinePromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pc3XB-ZF70
Plasmid#13193DepositorInsertZF70
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE
Plasmid#107723PurposeFor expression of ABE7.10 in mammalian cellsDepositorInsertABE7.10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
knob-s48i48
Plasmid#68391PurposeKnob protein fused to elastin-like polypeptide (S48I48)DepositorInsertKnob-S48I48
UseTagsKnobExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLPC-Myc-TZAP
Plasmid#87175Purposeretroviral stable expression of Myc-TZAPDepositorInsertTZAP (ZBTB48 Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_low
Plasmid#128851PurposeLow copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_med
Plasmid#128852PurposeMedium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only