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Addgene
Showing: 9221 - 9240 of 15934 results

  1. NET29A.Mm pEGFP-N2 (1326)

    Plasmid
    #62045
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29A (Tmem120a Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    62045-plasmid-map-sequence-id-101294

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  2. NET29b.Mm pEGFP-N2 (1327)

    Plasmid
    #62046
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29b (Tmem120b Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    62046-plasmid-map-sequence-id-101295

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  3. NET29A.Hs pRRLSIN (1742)

    Plasmid
    #62052
    Purpose
    lentiviral expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29 (TMEM120A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Availability
    Academic Institutions and Nonprofits only
    62052-plasmid-map-sequence-id-101296

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  4. pJK588

    Plasmid
    #72270
    Purpose
    Synthetic gene corresponding to Synechocystis PCC6803 orf sll1489
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein SYNGTS_3005
    Use
    Gene cloning
    Tags
    His6 and TEV cleavage site
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72270-plasmid-map-sequence-id-131740

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  5. pJK681

    Plasmid
    #72271
    Purpose
    Produces Synechocystis PCC6803 orf sll1489 (synthetic gene)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein SYNGTS_3005
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72271-plasmid-map-sequence-id-131742

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  6. p3m

    Plasmid
    #120935
    Purpose
    MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23150
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120935-plasmid-map-sequence-id-235944

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  7. p4m

    Plasmid
    #120936
    Purpose
    MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23151
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120936-plasmid-map-sequence-id-235945

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  8. C21m

    Plasmid
    #120962
    Purpose
    MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120962-plasmid-map-sequence-id-238043

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  9. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  10. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  11. C38m

    Plasmid
    #120971
    Purpose
    MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase active at transition from exponential phase to stationary phase). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 38 (RpoS)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120971-plasmid-map-sequence-id-236023

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  12. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  13. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

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  14. C82m

    Plasmid
    #120993
    Purpose
    MoClo golden gate assembly CD part for RpaI (synthase for Rpa-detected quorum sensing molecule 4-coumaroyl-homoserine lactone). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120993-plasmid-map-sequence-id-236098

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  15. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  16. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

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  17. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  18. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

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  19. U19m

    Plasmid
    #121024
    Purpose
    MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121024-plasmid-map-sequence-id-238066

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  20. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112558-plasmid-map-sequence-id-217235

    Enlarge

Showing: 9221 - 9240 of 15934 results