pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
(Plasmid
#52546)
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PurposeTargeting vector for homologous recombination in mouse ES cells; Rosa26 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRosa26
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namePhiC31o attPx3 (70 bp each)
Gene/Insert 2
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Gene/Insert nameNeo
Gene/Insert 3
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Gene/Insert namePhiC31o integrase
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPhiC31o from Phil Soriano
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5 was a gift from Liqun Luo (Addgene plasmid # 52546 ; http://n2t.net/addgene:52546 ; RRID:Addgene_52546) -
For your References section:
Site-specific integrase-mediated transgenesis in mice via pronuclear injection. Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L. Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 10.1073/pnas.1019507108. Epub 2011 Apr 4. 10.1073/pnas.1019507108 PubMed 21464299