Addgene

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pLEX305_3xFLAG_STAT1

Plasmid

#220323

Purpose

Expresses full length human STAT1 with an N-terminal 3xFLAG tag

Depositor

Jonathan Brown

Insert

STAT1 alpha (STAT1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

PGK

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP5

Plasmid

#22590

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP5 (USP5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP8

Plasmid

#22608

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP8 (USP8 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-PSMD14

Plasmid

#22557

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

PSMD14 (PSMD14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2B

Plasmid

#15781

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2B (UBE2B Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP10

Plasmid

#22543

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP10 (USP10 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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Active Wnt5A-V5

Plasmid

#43813

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT5A (WNT5A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt11-V5

Plasmid

#43824

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT11 (WNT11 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti PKAKTRn-Clover

Plasmid

#177200

Purpose

Lentiviral vector encoding PKAKTRnew-Clover

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Insert

PKAKTRnew-mClover

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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psgRNA

Plasmid

#114005

Purpose

express sgRNA. ColE1, Kan

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-HA-UCHL5

Plasmid

#22565

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

UCHL5 (UCHL5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Active Wnt7B-V5

Plasmid

#43817

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT7B (WNT7B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt2B-V5

Plasmid

#43808

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT2B (WNT2B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt9B-V5

Plasmid

#43821

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT9B (WNT9B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

M106T

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMOS017: ratiometric pHluorin (cytosolic)

Plasmid

#163052

Purpose

ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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Active Wnt3-V5

Plasmid

#43811

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT3 (WNT3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-HA-STAMBP

Plasmid

#22560

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

STAMBP (STAMBP Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pAC1801_pmax-dCasRx

Plasmid

#118634

Purpose

Transient transfection; Expresses dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Use

Tags

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Active Wnt3A-V5

Plasmid

#43810

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT3A (WNT3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge