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Addgene
Showing: 9201 - 9220 of 15840 results

  1. pmSod1-G93A-N’His

    Plasmid
    #117705
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    G93A
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    117705-plasmid-map-sequence-id-227526

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  2. phSOD1-E100G-N’His

    Plasmid
    #117706
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    E100G
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    117706-plasmid-map-sequence-id-227528

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  3. phSOD1-L126X-N’His

    Plasmid
    #117707
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    L126X
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    117707-plasmid-map-sequence-id-227541

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  4. pmSod1-Q42L-N’His

    Plasmid
    #117708
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    117708-plasmid-map-sequence-id-227535

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  5. pmSod1-Q123A-N’His

    Plasmid
    #117709
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q123A
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    117709-plasmid-map-sequence-id-227504

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  6. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

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  7. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Insert
    dCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

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  8. pcDNA3.1-hRIPK1-Flag-delN

    Plasmid
    #112492
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of N terminus (AA1-274 deleted)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112492-plasmid-map-sequence-id-214533

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  9. pcDNA3.1-hRIPK1-Flag-delC

    Plasmid
    #112493
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of C terminus (AA290-671 deleted)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112493-plasmid-map-sequence-id-214530

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  10. pcDNA3.1-hRIPK1-Flag-delDD

    Plasmid
    #112494
    Purpose
    Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of DD domain (AA584-671 deleted)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112494-plasmid-map-sequence-id-214528

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  11. thoc5.018.389

    Plasmid
    #137266
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    thoc5 (THOC5 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    018-389
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    137266-plasmid-map-sequence-id-323966

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  12. BFP-RanQ69L

    Plasmid
    #163438
    Purpose
    Mammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredation
    Depositor
    Sanford Simon
    Insert
    RanQ69L (RAN Human)
    Use
    Tags
    tag-BFP
    Expression
    Mammalian
    Mutation
    Q69L
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    163438-plasmid-map-sequence-id-321655

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  13. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Mouse Hsp68 minimal promoter
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  14. A-WDR5-1

    Plasmid
    #166515
    Purpose
    scFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
    166515-plasmid-map-sequence-id-327811

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  15. J-CDAN1A-5

    Plasmid
    #166522
    Purpose
    scFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
    166522-plasmid-map-sequence-id-328268

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  16. Z-MATR3-4

    Plasmid
    #166558
    Purpose
    scFv of a human scaffold targeting Matrin-3. Antigen coverage aa 390-578 of 847
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
    166558-plasmid-map-sequence-id-329266

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  17. Z-OPTN-10

    Plasmid
    #166562
    Purpose
    scFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
    166562-plasmid-map-sequence-id-327812

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  18. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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  20. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid
    #203653
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203653-plasmid-map-sequence-id-402484

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Showing: 9201 - 9220 of 15840 results