-
Plasmid#72833PurposeCRISPR/Cas to target the AAVS1 locus in human cellsDepositorInsertCas9 and gRNA for targeting the AAVS1 locus in human cells
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK287 (mAID-Hygro)
Plasmid#72825PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Hygro
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ECFPEYFP
Plasmid#24520DepositorInsertECFP, EYFP
UseTagsECFP and EYFPExpressionMammalianMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNP566
Plasmid#222724PurposepNP561 - IS621 WT Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fragment1 with TLS2
Plasmid#196983PurposeGateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.DepositorInsertIntermediate fragment for assembly of gRNA-TLS2 construct
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK281 (mCherry2-Hygro)
Plasmid#72797PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Hygro
UseCRISPRTagsmCherry2ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA Null
Plasmid#198728PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.DepositorInsertAtNIA1 gRNAs
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK288 (mAID-Bsr)
Plasmid#72826PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Bsr
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pMOD_C3010
Plasmid#91098PurposeModule C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3003
Plasmid#91095PurposeModule C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK278 (mClover-Hygro)
Plasmid#72794PurposeC-terminal tagging with mClover using CRIPSR/Cas9DepositorInsertmClover-Hygro
UseCRISPRTagsmCloverExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3001
Plasmid#91094PurposeModule C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fragment 1 Null
Plasmid#198729PurposeGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.DepositorInsertgRNA expression cassette in pENTR4
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SAC1C389SdeltaTMD-FKBP-mCherry
Plasmid#108124PurposeRecruitable cytosolic inactive SAC1DepositorInsertSACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(C389S; 1-520)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK282 (mCherry2-Bsr)
Plasmid#72798PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Bsr
UseCRISPRTagsmCherry2ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK279 (mClover-Bsr)
Plasmid#72795PurposeC-terminal tagging with mClover using CRIPSR/Cas9DepositorInsertmClover-Bsr
UseCRISPRTagsmCloverExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only