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Anti-QKI-7 [N183/15R]

Plasmid

#177507

Purpose

Mammalian Expression Plasmid of anti-QKI-7 (Human). Derived from hybridoma N183/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-7 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-BLAST EGFP-flag-CAD

Plasmid

#188118

Purpose

Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

EGFP tag and Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ Channel [D3/71R]

Plasmid

#128612

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ Channel (Rat). Derived from hybridoma D3/71.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ Channel (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FlpO-bGHpA

Plasmid

#51669

Purpose

Can be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV Retrograde

Insert

FlpO recombinase gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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MEK1 (S218D,S222D)-pcw107

Plasmid

#64604

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S218D, S222D

Promoter

PGK

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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MEK5 DD(S311D,T315D)-pcw107-V5

Plasmid

#64619

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

S311D, T315D*

Promoter

PGK

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
Anti-IRSp53/BAIAP2 [L117/1R]

Plasmid

#177462

Purpose

Mammalian Expression Plasmid of anti-IRSp53/BAIAP2 (Human). Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-IRSp53/BAIAP2 (Homo sapiens) recombinant mouse monoclonal antibody (BAIAP2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag YES1

Plasmid

#20665

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

YES1 (YES1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
px330-Axin1

Plasmid

#162543

Purpose

sgRNA targeting Axin1

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Axin1 (Axin1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

Plasmid

#127660

Purpose

Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mutation

E225A/D227A

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Hras (G12V)-pcw107

Plasmid

#64603

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (HRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
Anti-PSD-95 [K28/43R]

Plasmid

#128618

Purpose

Mammalian Expression Plasmid of anti-PSD-95 (Human). Derived from hybridoma K28/43.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

Anti-PSD-95 (Human) recombinant mouse monoclonal antibody (DLG4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mCherry-FLAG-cGAS E225A/D227A

Plasmid

#127657

Purpose

Over-express catalytically inactive mCherry-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Flag and mCherry

Expression

Mutation

E225A/D227A

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Lgi1 [N283/7R]

Plasmid

#177527

Purpose

Mammalian Expression Plasmid of anti-Lgi1 (Mouse). Derived from hybridoma N283/7R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Lgi1 (Mus musculus) recombinant mouse monoclonal antibody (Lgi1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST Halo-flag-CAD

Plasmid

#188117

Purpose

Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag and Halo tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Bassoon scFv [L124/59]

Plasmid

#182078

Purpose

Mammalian Expression of Bassoon scFv. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Bassoon (Rattus norvegicus) (Bsn Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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p38 WT O/E (MAPK14)-pcw107-V5

Plasmid

#64624

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK14 (transcript variant 2) (MAPK14 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
AR-V7-pcw107

Plasmid

#64635

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

splice isoform*

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
Anti-GFAP [N206A/8R-2b]

Plasmid

#199410

Purpose

Mammalian expression plasmid of Anti-GFAP [N206A/8R-2b] (Human). Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFAP (Human) recombinant mouse monoclonal antibody (GFAP Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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