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Plasmid#176267PurposeThis AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.DepositorInsertFlex-P301L tau (MAPT )
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PLK1-N-16
Plasmid#55119PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorInsertPLK1 (PLK1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2
Plasmid#205161Purposeexpress β-ACTIN-3′UTR-24xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg2b
Plasmid#127155PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotypeDepositorInsertIghg2b (Ighg2b Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP
Plasmid#40640DepositorInsertNICD3 (NOTCH3 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64
Plasmid#85423PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-5 synNotch
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51085-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
UseTagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a_IM
Plasmid#84478PurposeLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)DepositorInsertdCas9-Dnmt3a_IM
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5-39
Plasmid#118088PurposeFKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmidDepositorInsertFKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE
Plasmid#123363PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.DepositorInsertEGFP-NLS
UseCRISPR, Lentiviral, and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-mCherry
Plasmid#174188PurposeExpress mCherry with mitochondria-targeting sequence in mammalian cellsDepositorInsertmCherry with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP71-HLA-A0201-His
Plasmid#108214PurposeHLA-A0201 his taggedDepositorInsertmajor histocompatibility complex, class I, B (HLA-A Human)
UseRetroviralTagshisExpressionMammalianMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK1tight
Plasmid#89362PurposeA lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cellsDepositorInsertpsMEK1tight (MAP2K1 Synthetic, Human)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pERM2-nhpSer
Plasmid#201922PurposeEnables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.DepositorInsertsSepRS(2)
Sep-tRNA v2.0
SerB
EF-Sep
UseTagsNoneExpressionBacterialMutationE412P, E414F, T417K, P495M, I496W, F529S and H67R…PromoterGlnS (constitutive), OXB20 (constitutive), lpp (c…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-C1
Plasmid#36412DepositorTypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
CD-MPR-GFP-RUSH
Plasmid#202797PurposeRUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPRDepositorInsertM6PR (M6PR Human)
UseTagsEGFPExpressionMammalianMutationChanged 40 K (AAA) with R (AGA)PromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only